Canine bartonellosis: serological and molecular prevalence in Brazil and evidence of co-infection with Bartonella henselae and Bartonella vinsonii subsp. berkhoffii

Intracellular Pathogens Research Laboratory, Center for Comparative Medicine and Translational Research, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA.
Veterinary Research (Impact Factor: 2.82). 09/2007; 38(5):697-710. DOI: 10.1051/vetres:2007023
Source: PubMed


The purpose of this study was to determine the serological and molecular prevalence of Bartonella spp. infection in a sick dog population from Brazil. At the São Paulo State University Veterinary Teaching Hospital in Botucatu, 198 consecutive dogs with clinicopathological abnormalities consistent with tick-borne infections were sampled. Antibodies to Bartonella henselae and Bartonella vinsonii subsp. berkhoffii were detected in 2.0% (4/197) and 1.5% (3/197) of the dogs, respectively. Using 16S-23S rRNA intergenic transcribed spacer (ITS) primers, Bartonella DNA was amplified from only 1/198 blood samples. Bartonella seroreactive and/or PCR positive blood samples (n=8) were inoculated into a liquid pre-enrichment growth medium (BAPGM) and subsequently sub-inoculated onto BAPGM/blood-agar plates. PCR targeting the ITS region, pap31 and rpoB genes amplified B. henselae from the blood and/or isolates of the PCR positive dog (ITS: DQ346666; pap31 gene: DQ351240; rpoB: EF196806). B. henselae and B. vinsonii subsp. berkhoffii (pap31: DQ906160; rpoB: EF196805) co-infection was found in one of the B. vinsonii subsp. berkhoffii seroreactive dogs. We conclude that dogs in this study population were infrequently exposed to or infected with a Bartonella species. The B. henselae and B. vinsonii subsp. berkhoffii strains identified in this study are genetically similar to strains isolated from septicemic cats, dogs, coyotes and human beings from other parts of the world. To our knowledge, these isolates provide the first Brazilian DNA sequences from these Bartonella species and the first evidence of Bartonella co-infection in dogs.

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Available from: Ricardo G Maggi, Oct 05, 2015
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    • "DNA was extracted using a QIAxtractor (QIAGEN, Valencia, CA) following the tissue protocol provided by the manufacturer. A PCR assay was used to detect the presence of Bartonella DNA by amplification of the citrate synthase gene (gltA), using primers CS443f and CS1210r, as described by Billeter et al. 2011, and the 16S–23S intergenic spacer region (ITS) using primers 325f and 1100r as described by Diniz et al. 2007. Nuclease free water was used as a negative control and Bartonella doshiae DNA as a positive control. "
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    ABSTRACT: In the present study, we investigated 238 fleas collected from cats and dogs in three regions of Peru (Ancash, Cajamarca, and Lima) for the presence of Bartonella DNA. Bartonella spp. were detected by amplification of the citrate synthase gene (16.4%) and the 16S-23S intergenic spacer region (20.6%). Bartonella rochalimae was the most common species detected followed by Bartonella clarridgeiae and Bartonella henselae. Our results demonstrate that dogs and cats in Peru are infested with fleas harboring zoonotic Bartonella spp. and these infected fleas could pose a disease risk for humans.
    Journal of Medical Entomology 09/2015; DOI:10.1093/jme/tjv137 · 1.95 Impact Factor
    • "The presence of Anaplasma spp./Ehrlichia spp., Babesia spp., Bartonella spp., Bo. burgdorferi s.l., Cercopithifilaria spp., Hepatozoon spp., L. infantum, and Rickettsia spp. DNA in ticks was tested by PCR, with the primers and PCR conditions described in Table 1, according to previously described protocols (Regnery et al., 1991; Schwartz et al., 1992; Cortes et al., 2004; Diniz et al., 2007; Harrus et al., 2011; Otranto et al., 2011). PCR amplifications were performed in a 25-l final volume reaction containing 2 mM MgCl 2 , 1 unit of Taq DNA polymerase (GoTaq DNA Polymerase ® , Promega, USA), 10 pmol of each primer (15 pmol and 50 pmol in the case of Leishmania spp. "
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    ABSTRACT: Ticks are important vector arthropods of human and animal pathogens. As information about agents of disease circulating in vectors in Portugal is limited, the aim of the present study was to detect bacteria and parasites with veterinary and zoonotic importance in ticks collected from dogs, cats, and field vegetation. A total of 925 ticks, comprising 888 (96.0%) adults, 8 (0.9%) nymphs, and 29 (3.1%) larvae, were collected in 4 geographic areas (districts) of Portugal. Among those, 620 (67.0%) were removed from naturally infested dogs, 42 (4.5%) from cats, and 263 (28.4%) were questing ticks obtained from field vegetation. Rhipicephalus sanguineus was the predominant tick species, and the only one collected from dogs and vegetation, while all Ixodes ricinus specimens (n = 6) were recovered from cats. Rickettsia massiliae and Rickettsia conorii were identified in 35 ticks collected from cats and dogs and in 3 ticks collected from dogs. Among ticks collected from cats or dogs, 4 Rh. sanguineus specimens were detected with Hepatozoon felis, 3 with Anaplasma platys, 2 with Hepatozoon canis, one with Anaplasma phagocytophilum, one with Babesia vogeli, one with Borrelia burgdorferi sensu lato and one with Cercopithifilaria spp. Rickettsia helvetica was detected in one I. ricinus tick collected from a cat. To the best of our knowledge, this was the first time that Cercopithifilaria spp., Ba. vogeli, H. canis, and H. felis have been detected in ticks from Portugal. The wide range of tick-borne pathogens identified, some of zoonotic concern, suggests a risk for the emergence of tick-borne diseases in domestic animals and humans in Portugal. Further studies on these and other tick-borne agents should be performed to better understand their epidemiological and clinical importance, and to support the implementation of effective control measures.
    Ticks and Tick-borne Diseases 06/2014; 5(4). DOI:10.1016/j.ttbdis.2014.01.009 · 2.72 Impact Factor
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    • "during three independent steps: PCR from the original specimen prior to enrichment culture, PCR from the BAPGM liquid medium after enrichment culture, and PCR from subculture agar plate isolates, if obtained, [15] [16]. Briefly, a portion of each original specimen (2 ml of aseptically-obtained ethylenediaminetetraacetic acid (EDTA)-anticoagulated blood or 200 ␮l to 2 ml of a diagnostic fluid specimen diluted proportionally to maintain a 1:5 ratio of specimen to media) was inoculated into liquid medium (BAPGM) and incubated as previously described. "
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