Discrimination and Selective Enhancement of Signals in the MALDI Mass Spectrum of a Protein by Combining a Matrix-Based Label for Lysine Residues with a Neutral Matrix

Protein Mass Spectrometry Laboratory, Institut de Biologie Structurale, CEA, CNRS, UJF, UMR 5075, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France.
Angewandte Chemie International Edition (Impact Factor: 11.34). 07/2007; 46(29):5594-7. DOI: 10.1002/anie.200700811
Source: PubMed

ABSTRACT Discrimination with a difference: The N-hydroxysuccinimide ester of HCCA (α-cyano-4-hydroxycinnamic acid) was used as a labeling reagent to increase the MALDI MS signal of weakly concentrated peptides of interest in a proteolytic mixture derived from cytochrome c relative to the signals of other peptides. The desired effect was only observed with the neutral MALDI matrix HCCE.