Compositional analysis of water-soluble materials in corn stover.

Department of Chemistry & Biochemistry, Baylor University, One Bear Place, Box 97348, Waco, Texas 76798, USA.
Journal of Agricultural and Food Chemistry (Impact Factor: 3.11). 08/2007; 55(15):5912-8. DOI: 10.1021/jf0700327
Source: PubMed

ABSTRACT Corn stover is one of the leading feedstock candidates for commodity-scale biomass-to-ethanol processing. The composition of water-soluble materials in corn stover has been determined with greater than 90% mass closure in four of five representative samples. The mass percentage of water-soluble materials in tested stover samples varied from 14 to 27% on a dry weight basis. Over 30 previously unknown constituents of aqueous extracts were identified and quantified using a variety of chromatographic techniques. Monomeric sugars (primarily glucose and fructose) were found to be the predominant water-soluble components of corn stover, accounting for 30-46% of the dry weight of extractives (4-12% of the dry weight of feedstocks). Additional constituents contributing to the mass balance for extractives included various alditols (3-7%), aliphatic acids (7-21%), inorganic ions (10-18%), oligomeric sugars (4-12%), and a distribution of oligomers tentatively identified as being derived from phenolic glycosides (10-18%).

  • [Show abstract] [Hide abstract]
    ABSTRACT: A method was developed for simultaneous determination of seven sugar compounds and two uronic acids by high-performance anion exchange chromatography (HPAEC) with pulsed amperometric detection (PAD) using a CarboPac PA20 column under a gradient elution (mobile phase: H2O/200 mM NaOH/100 mM NaAc) at 0.4 mL/min. Under these conditions, d-(−)-arabinose, d-(+)-galactose, d-(+)-glucose, d-(+)-mannose, d-(+)-xylose, d-(−)-fructose, d-(+)-cellobiose, d-galacturonic acid, and d-glucuronic acid were all separated in 46 min. The calibration curve, detection limits, reproducibility and accuracy were also determined. Furthermore, seven sugar compounds and two uronic acids were all detected in the enzymatic hydrolysates of different lignocellulosic materials treated by different pretreatment processes. Our results demonstrate that this method was feasible for sugar compounds separation without the need for derivatization and organic solvent, and it also has the advantages of having low interference, high sensitivity, low detection limit of picomol, and excellent reproducibility and accuracy.
    Energy & Fuels 04/2012; 26(5):2942–2947. · 2.73 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Solid-state anaerobic digestion (SS-AD) and composting of yard trimmings with effluent from liquid AD were compared under thermophilic condition. Total solids (TS) contents of 22%, 25%, and 30% were studied for SS-AD, and 35%, 45%, and 55% for composting. Feedstock/effluent (F/E) ratios of 2, 3, 4, 5, and 6 were tested. In composting, the greatest carbon loss was obtained at 35% TS, which was 2-3 times of that at 55% TS and was up to 50% higher than that in SS-AD. In SS-AD, over half of the degraded carbon was converted to methane with the greatest methane yield of 121L/kgVSfeedstock. Methane production from SS-AD was low at F/E ratios of 2 and 3, likely due to the inhibitory effect of high concentrations of ammonia nitrogen (up to 5.6g/kg). The N-P-K values were similar for SS-AD digestate and compost with different dominant nitrogen forms.
    Bioresource Technology 07/2014; 169C:439-446. · 5.04 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Spent mushroom substrate (SMS) is a biomass waste generated from mushroom production. About 5kg of SMS is generated for every kg of mushroom produced. In this study, solid state anaerobic digestion (SS-AD) of SMS, wheat straw, yard trimmings, and their mixtures was investigated at different feedstock to effluent ratios. SMS was found to be highly degradable, which resulted in inhibition of SS-AD due to volatile fatty acid (VFA) accumulation and a decrease in pH. This issue was addressed by co-digestion of SMS with either yard trimmings or wheat straw. SS-AD of SMS/yard trimmings achieved a cumulative methane yield of 194L/kg VS, which was 16 and 2 times higher than that from SMS and yard trimmings, respectively. SS-AD of SMS/wheat straw obtained a cumulative methane yield of 269L/kg VS, which was 23 times as high as that from SMS and comparable to that from wheat straw.
    Bioresource Technology 07/2014; 169C:468-474. · 5.04 Impact Factor