Article

Phytase expression in transgenic soybeans: stable transformation with a vector-less construct.

Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian, 116024, P.R. China.
Biotechnology Letters (impact factor: 1.68). 12/2007; 29(11):1781-7. DOI:10.1007/s10529-007-9439-x pp.1781-7
Source: PubMed

ABSTRACT A minimal linear gene cassette (35S-phytase gene-nos) with T-DNA borders was acquired by PCR and directly introduced into soybean through the pollen tube pathway. A total of 13% of T(1 )plants were positive for phyA by specific PCR. Southern blot analyses showed that phyA insertions were harbored stably in T(2) progeny. Phytase expression level increased 2.5-fold over a 6-week period; its highest activity was 150 U/mg protein, compared to 56 U/mg protein in untransformed controls. Activity of phytase increased to 125 FTU/kg in T(3) transgenic seeds as compared to 64 FTU/kg in wild-type plants.

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Keywords

35S-phytase gene-nos
 
highest activity
 
minimal linear gene cassette
 
phytase
 
Phytase expression level
 
pollen tube pathway
 
soybean
 
specific PCR
 
T-DNA borders
 

Xiao-Rong Gao