Article

Immunoglobulin E (IgE)-mediated cross-reactivity between mesquite pollen proteins and lima bean, an edible legume.

Institute of Genomics and Integrative Biology, Delhi, India.
Clinical & Experimental Immunology (impact factor: 3.36). 10/2007; 149(3):517-24. DOI:10.1111/j.1365-2249.2007.03446.x pp.517-24
Source: PubMed

ABSTRACT Immunoglobulin E (IgE)-mediated food allergy often develops as a consequence of allergic sensitization to pollen proteins. Mesquite (Prosopis juliflora) tree pollen is reported to be cross-reactive with other pollen species, but little has been reported on its cross-reactivity with plant-derived foods belonging to the same/different families. The present study investigates the in vitro cross-reactivity of mesquite pollen and lima bean (Phaseolus lunatus), an edible seed belonging to the Leguminosae family. Of 110 patients (asthma, rhinitis or both) tested intradermally, 20 showed marked positive reactions with Prosopis pollen extract. Of these, 12 patients showed elevated specific IgE to Prosopis pollen extract alone and four to both Phaseolus and pollen extract. In vitro cross-reactivity was investigated using inhibition assays [enzyme-linked immunosorbent assay (ELISA) inhibition, immunoblot inhibition], histamine release and lymphoproliferation. P. lunatus extract could inhibit IgE binding to P. juliflora in a dose-dependent manner, requiring 400 ng of protein for 50% inhibition in ELISA assay. Immunoblot and immunoblot inhibition demonstrated the presence of 20, 26, 35, 66 and 72 kDa as shared IgE binding components between the two extracts. Histamine release, peripheral blood mononuclear cells proliferation and interleukin (IL)-4 levels also suggested allergenic cross-reactivity. In conclusion, there is humoral and cellular cross-reactivity between Prosopis pollen and Phaseolus seed allergens.

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Keywords

allergenic cross-reactivity
 
allergic sensitization
 
cellular cross-reactivity
 
IgE binding
 
IgE binding components
 
IgE)-mediated food allergy
 
immunoblot inhibition]
 
lima bean
 
mesquite pollen
 
P. juliflora
 
peripheral blood mononuclear cells proliferation
 
plant-derived foods
 
pollen proteins
 
pollen species
 
present study investigates
 
Prosopis juliflora
 
Prosopis pollen
 
same/different families
 
specific IgE
 
vitro cross-reactivity