A new cytotoxic phenazine derivative from a deep sea bacterium Bacillus sp

Key Laboratory of Marine Drugs, Chinese Ministry of Education, Institute of Marine Drugs and Food, Ocean University of China, Qingdao 266003, PR China.
Archives of Pharmacal Research (Impact Factor: 2.05). 06/2007; 30(5):552-5. DOI: 10.1007/BF02977647
Source: PubMed


A novel phenazine derivative (1) together with six known compounds (2-7) were isolated by bioassay-guided fractionation from the culture broth of a bacterium, Bacillus sp., collected from a Pacific deep sea sediment sample (depth 5059 m). The structures of these compounds were determined using spectroscopic methods. Their cytotoxic effects on P388 and K562 cell lines were preliminarily examined using the sulforhodamine-B (SRB) assay.

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Available from: Dehai Li, May 15, 2014
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    • "The phenazine antibiotics isolated from Bacillus sp. of Pacific deep-sea sediment (depth of 5059 m) possessed anticancer activity on P388 cancer cell line. The antibiotics such as 5,10- dihydrophencomycin methyl ester and phencomycin (isolated from an unidentified marine Streptomyces sp.) [23] have weak activity against E. coli and B. subtilis [24]. N-(2-hydroxyphenyl)-2-phenazinamine isolated from marine actinomycete (BM-17) has cytotoxicity against K562, HepG2, MGC803, HCT116 and MCF7 cancer cell lines, as well as 293T non-cancer cell line [25]. "
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    • "Lately, Homann et al. reported the isolation of peptidic siderophores Loihichelins A-F [8] from the cultures of heterotrophic bacterium Halomonas LOB-5 collected from the southern rift zone of Loihi Seamount east of Hawaii. Novel cytotoxic phenazine derivatives [9] with unique ring system have been described from pacific sediment Bacillus sp. (5059 m depth). "
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    • "Structural investigations identified the two pigments as novel phenazostatin D, inactive against the tested microorganisms, and methyl saphenate, a known phenazine antibiotic. Li et al. [64] also reported the isolation of a novel phenazine derivative with cytotoxic effects against P388 cells, together with six previously identified compounds from the marine Bacillus sp., collected from a Pacific deep-sea sediment sample at a depth of 5059 m. A novel phenazine derivative with antibiotic activity, identified as 5,10-dihydrophencomycin methyl ester, along with (2-hydroxyphenyl)-acetamide, menaquinone MK9 (II, III, VIII, IX-H8), and phencomycin, was isolated from an unidentified marine Streptomyces sp. by Pusecker et al. [65]. "
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    ABSTRACT: Research into natural products from the marine environment, including microorganisms, has rapidly increased over the past two decades. Despite the enormous difficulty in isolating and harvesting marine bacteria, microbial metabolites are increasingly attractive to science because of their broad-ranging pharmacological activities, especially those with unique color pigments. This current review paper gives an overview of the pigmented natural compounds isolated from bacteria of marine origin, based on accumulated data in the literature. We review the biological activities of marine compounds, including recent advances in the study of pharmacological effects and other commercial applications, in addition to the biosynthesis and physiological roles of associated pigments. Chemical structures of the bioactive compounds discussed are also presented.
    Evidence-based Complementary and Alternative Medicine 09/2011; 2011(3):670349. DOI:10.1155/2011/670349 · 1.88 Impact Factor
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