Donor Myocardial HIF-1? Is an Independent Predictor of Cardiac Allograft Dysfunction: A 7-Year Prospective, Exploratory Study

Department of Cardiothoracic Surgery, Medical University of Vienna, Vienna, Austria.
American Journal of Transplantation (Impact Factor: 5.68). 09/2007; 7(8):2012-9. DOI: 10.1111/j.1600-6143.2007.01875.x
Source: PubMed


Knowledge on interplay between the cardiac molecular response to transplantation-induced stress and primary graft dysfunction (PGD) is limited. A cDNA array identified HIF-1, EGR-1, NAB-2, VEGF-A and uPA as mediators of cardiac tissue response to transplantation-induced stress. mRNA expression of these molecules was measured in left ventricular biopsies from 200 donors before and after aortic cross-clamping and at 10-, 30- and 60-min reperfusion by real-time RT-PCR. HIF-1alpha expression at two time points was significantly associated with PGD, as shown by univariate analysis, receiver operating characteristic curve and multivariate logistic regression. At a cut-off level of 200 arbitrary units, HIF-1alpha after aortic cross-clamping in donors (78% sensitivity, 83% specificity) and at 10-min reperfusion (85% sensitivity, 83% specificity) identified PGD. HIF-1alpha demonstrates the potential to be a predictive marker for PGD; however, as multiple factors were tested at different time points, prospective evaluation is clearly necessary to confirm this observation.

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Available from: Georg Wieselthaler, May 26, 2015
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    • "It affects an estimated 10 to 25% of all lung transplants [5]. Ischemia reperfusion injury is known to prime transplanted organs to be more susceptible for later rejection episodes [6–8]. Preventing the occurrence of such reperfusion damages might be an important therapeutic strategy in conserving the organ’s long-term function [5]. "
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