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Analysis of Rhizoma Polygoni Cuspidati by HPLC and HPLC-ESI/MS

West China School of Pharmacy, Sichuan University, No. 17 Section 3 Ren-Min-Nan-Lu Road, Chengdu, Sichuan 610041, People's Republic of China.
Phytochemical Analysis (Impact Factor: 2.45). 09/2007; 18(5):387-92. DOI: 10.1002/pca.993
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ABSTRACT An HPLC method with photodiode array detection (PAD) and ESI/MS detection was developed for the qualitative and quantitative analysis of the major chemical constituents of the dried rhizome of Polygonum cuspidatum Sieb. et Zucc. (Rhizoma Polygoni Cuspidati; Chinese name Hu-Zhang). Based on the chromatographic separation on an Altima C(18) column using 0.5% aqueous acetic acid and acetonitrile as the mobile phase, nine compounds, including stilbenes, stilbene glucosides, anthraquinones and anthraquinone glucosides, were identified by online ESI/MS analysis and seven were quantified by HPLC-PAD. A full validation of the method including sensitivity, linearity, repeatability and recovery was conducted. Linear calibration was achieved over the concentration range 1-200 mg/L with R(2) > 0.999, whilst the limits of detection ranged from 0.51 to 1.57 ng. Repeatability was evaluated by intra- and inter-day assays and the RSD value was within 1.79%. Recoveries of the quantified compounds were within the range 96.0-100.1% with RSD values of less than 2.2%. Five samples of Rhizoma Polygoni Cuspidati from different regions were analysed using the developed method. The major constituents piceid, resveratrol, emodin-8-O-beta-D-glucoside and emodin were selected to provide an index for the quality assessment of the herbal drug.

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    • "). P. cuspidatum is a medicinal plant that has been widely used in Chinese and Japanese folk medicine for the treatment of atherosclerosis, hypertension, cough, suppurative dermatitis, and gonorrhea (Yi et al. 2007). Various aromatic stilbenes including resveratrol , piceid and 2,3,5,4 0 -tetrahydroxystilbene-2-O-D- glucoside, have been isolated from this plant (Xiao et al. 2002; Hegde et al. 2004), suggesting that, besides the typical CHS involved in the biosynthesis of flavonoids, the STS is also present in P. cuspidatum. "
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    ABSTRACT: In our recent work (Ma et al., in Planta 229(3):457–469, 2009a and 229(4):1077–1086, 2009b), two three-intron type III PKS genes, PcPKS1 and PcPKS2, were isolated from Polygonum cuspidatum Sieb. et Zucc. Phylogenetic and functional analyses revealed PcPKS1 is a three-intron chalcone synthase (CHS) gene, and PcPKS2 is found to be a three-intron benzalacetone synthase (BAS) gene. The regular CHS encoded by a single intron gene have not been isolated and characterized from P. cuspidatum. In this work a further CHS with one intron (PcPKS3) and a stilbene synthase (STS) gene with three-intron (PcPKS5) were isolated and characterized by functional and phylogenetic analyses. In comparison with PcPKS1, a bifunctional enzyme with both CHS and BAS activity, the enzymatic product of recombinant PcPKS3 was naringenin, bis-noryangonin (BNY) and 4-coumaroyltriacetic acid lactone (CTAL) occurred as side products. The PcPKS5 synthesized resveratrol and a trace amount of naringenin from p-coumaroyl-CoA. To our knowledge, PcPKS5 is the first reported three-intron STS gene in flowering plants. In this work, we speculated that this involved a possible evolutionary route of plant-specific type III PKS superfamily in P. cuspidatum.
    Plant Biotechnology Reports 07/2013; 7(3). DOI:10.1007/s11816-012-0271-y · 1.59 Impact Factor
    • "No piceatannol was detected in the roots of P. cuspidatum from China, but could be found obviously in P. cuspidatum from Switzerland; meanwhile, the relative contents of resveratroloside and some proanthcyanidins increased.[4] It is generally accepted that multiple constituents are responsible for the therapeutic effect of plants.[5] Can the invasive variety present in Switzerland still be used like the native traditional medicine from China? "
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    ABSTRACT: Polygonum cuspidatum Sieb. and Zucc. has been traditionally used as a member of many anti-inflammatory polyherbal formulations, but is now a widespread invasive neophyte in Europe and America. To discuss if the invasive variety is chemically identical to the native one in traditional medicine, the different constituents of the invasive variety compared to the native variety were isolated and their anti-inflammatory activity was tested. Resveratroloside and catechin-(4α→8)-catechin, the newly found constituents in the invasive variety, have similar nitric oxide (NO) inhibition potency as that of piceid (the major constituent of P. cuspidatum), but the newly found major constituent, i.e., piceatannol glucoside, showed no apparent effect. On the other hand, as a marker, the total content of resveratrol in the methanol root extract after glucosidase hydrolysis was measured and compared between the invasive and native varieties. The total content of resveratrol measured in the root extracts of the Swiss sample was about 2.5 times less than that of the Chinese one. This study brings attention to the point that when the invasive variety of P. cuspidatum is used in traditional medicine, the chemical difference should be kept in mind.
    07/2013; 3(3):182-7. DOI:10.4103/2225-4110.114905
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    • "et Zucc. (Polygonaceae) is a medicinal plant that has been widely used in Chinese and Japanese folk medicine for the treatment of atherosclerosis, hypertension, cough, suppurative dermatitis, and gonorrhea (Yi et al. 2007). Various aromatic polyketides including anthraquinones, such as emodin, physcion and chrysophanol , and stilbenes, such as resveratrol, piceid and 2,3,5,4′- tetrahydroxystilbene-2-O-D-glucoside, have been isolated from this plant (Xiao et al. 2002; Hegde et al. 2004), suggesting that, besides the typical CHS involved in the biosynthesis of flavonoids, several functionally divergent PKSs occur in P. cuspidatum. "
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    ABSTRACT: By using the TAIL-PCR and over-hang extension PCR methods, we successfully isolated a three-intron type III PKS gene and cDNA clone from the Polygonum cuspidatum Sieb. et Zucc, and named it PcPKS4. The phylogenetic analysis showed that PcPKS4 is located in a cluster containing all functionally divergent CHS-like enzymes from angiosperms. Interestingly, the CHS “gatekeeper” phenylalanine, Phe265 is uniquely replaced by Leu in PcPKS4. Different from our previously results, RNA gel–blot analysis revealed that the PcPKS4 transcripts were present at a low level in roots.
    Journal of Plant Biochemistry and Biotechnology 01/2012; 23(1). DOI:10.1007/s13562-012-0184-4 · 0.81 Impact Factor
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