IPG with electrodic plateaus (and other unusual procedures for 2-DE).

Gruppo di Studio per la Proteomica e la Struttura delle Proteine, Dipartimento di Scienze Farmacologiche, Università degli Studi, Milano, Italia.
Electrophoresis (Impact Factor: 3.26). 09/2007; 28(16):2953-6. DOI: 10.1002/elps.200600722
Source: PubMed

ABSTRACT We describe some simple changes to the geometry of the IPG strips that make them suitable to the loading of very large sample volumes and of high-salt solutions. Of special relevance is the possibility of using strips with immobilized plateau(s) to either side of the gradient, or to both, also in connection with in-gel rehydration protocols and focusing in stock trays. The only requirement to achieve this is to leave the all-ready-made attitude and go back to custom polymerization of the IPGs in one's laboratory.

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