Transplantation of germ cells and testis tissue for the study and preservation of fertility.
ABSTRACT The germ line stem cells in the mammalian testis form the basis of male fertility. When these stem cells are transplanted from the testis of a fertile donor animal to the testis of an infertile recipient they can establish donor-derived spermatogenesis in the recipient testis, and the resulting sperm can transmit the genotype of the donor to the offspring of the recipient. Germ cell transplantation provides a system to study the biology of germ line stem cells, to explore stem cell isolation and culture, to examine defects in spermatogenesis and to overcome male infertility. Although most widely studied in rodents, germ cell transplantation is now applied to larger mammals, including primates. Germ cell transplantation can preserve fertility from valuable animals and potentially restore fertility in patients that underwent cytotoxic treatments for cancer. In addition, genetic manipulation of germ cells prior to transplantation provides a new approach to germ line modification and transgenesis. As an alternative to transplantation of isolated germ cells to a recipient testis, ectopic grafting of testis tissue from diverse mammalian donor species, including primates, into a mouse host represents a model to study spermatogenesis, to investigate the effects of substances with the potential to enhance or suppress male fertility, and to produce fertile sperm from immature donors. Therefore, transplantation of germ cells or testis tissue are uniquely valuable approaches for the study, preservation and manipulation of male fertility in mammalian species.
SourceAvailable from: Xingbo Xu[Show abstract] [Hide abstract]
ABSTRACT: Stem cells have the capacity for self-renewal and the ability to differentiate to various cell lineages. Thus, they represent an important building block for regenerative medicine and tissue engineering. Current research focuses on the possible exploitation of stem cells in medicine and their potential to offer a range of effective treatments for various diseases. A variety of stem cells, ranging from embryonic, bone marrow, endogenous, and amniotic fluid have been investigated and may prove useful as novel alternatives for organ regeneration both in vitro and in vivo. ESCs are pluripotent cells derived from the inner cell mass of the early mammalian embryo. Because of their plasticity and potentially unlimited capacity for self-renewal, ESCs have generated tremendous interest both as models for developmental biology and as possible tools for regenerative medicine. This excitement has been attenuated, however, by scientific, political, and ethical considerations. To exploit this potential, it is essential to be able to control ESC differentiation and to direct the development of these cells along specific pathways. Embryology has offered important insights into key pathways regulating ESC differentiation, resulting in advances in modeling gastrulation in vitro and in the efficient induction of endoderm, mesoderm, and ectoderm, and many of their downstream derivatives. This has led to the identification of new multipotential progenitors for the hematopoietic, neural, and cardiovascular lineages and to the development of protocols for the efficient generation of a broad spectrum of cell types including hematopoietic cells, cardiomyocytes, oligodendrocytes, dopamine neurons, and immature pancreatic β- cells. The next challenge will be to demonstrate the functional utility of these cells, both in vitro and in preclinical models of human disease. Read More: http://informahealthcare.com/doi/abs/10.3109/9781841847290.010Stem Cells in Human Reproduction, Second Edition 09/2009: chapter Chapter 10: Stem Cell–Based Therapeutic Approaches for Treatment of Male Infertility;
[Show abstract] [Hide abstract]
ABSTRACT: This review describes the regulation of spermatogenesis taking into consideration the hypothalamic-pituitary gonadal axis, the male reproductive organs and the endocrine and paracrine factors involved in the control of sperm production and the release of androgens. Instead of detailed descriptions of many hormones and growth factors, we attempt to provide an integrative and evolutionary view by comparing different species and considering their specific needs for successful male reproduction. The review focuses on species specific differences in the structural organization of spermatogenesis and indicates that the crucial regulatory mechanisms controlling sperm output are targeted towards differentiating spermatogonia when they initiate clonal expansion. We argue that the further differentiation of germ cells is following a highly coordinated and strictly predetermined morphogenetic cascade widely independent of hormonal control. We propose a hypothetical "ancient" model. Spermatogenesis and steroidogenesis are controlled by a master switch (GnRH pulse generator) under whose control two separate feedback systems provide independent control of androgen (LH-testosterone) and sperm production (FSH-inhibin). This scenario offers high flexibility and has seen uncountable adaptions to optimize the specific needs of different species. Models for the hormonal regulation in hamsters, laboratory rodents and primates are presented to illustrate the species specific diversity.Seminars in Cell and Developmental Biology 03/2014; 29. DOI:10.1016/j.semcdb.2014.03.007 · 5.97 Impact Factor
[Show abstract] [Hide abstract]
ABSTRACT: In cattle,assisted reproductive technologies (ART) can be defined as techniques that manipulate reproductive-related events and/or structures to achieve pregnancy with the final goal of producing healthy offspring in bovine females. The present review includes manipulation of female reproductive tract physiology, artificial insemination, multiple ovulation and embryo transfer, in vitro production of embryos, in vitro assisted fertilization, cloning, transgenesis, xenografting-germ cell transplantation, preimplantation genetic diagnosis and sperm sexing. This review shows that several ART are being currently applied commercially in the cattle industry with acceptable results. On the other hand, others have low efficiency in producing cattle offspring and are predominantly applied in experimental settings. Several of these ART can cause detrimental effects at the prenatal and postnatal period and therefore they need to be improved. However, even if thesebovine-related biotechnologies are properly improved, they might be more useful in the conservation of endangered ungulates, production of pharmaceuticals, or as experimental models for human reproduction.02/2008; DOI:10.5016/1806-8774.2008.v10p36