Article

[Effect of GMCSF-absence on neovascularization during wound healing].

Burns & Plastic Surgery Department, the Third People Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 201900, China.
Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery 06/2007; 23(3):233-5. pp.233-5
Source: PubMed

ABSTRACT To study the effect of GMCSF-absence on the rate of wound healing and neovascularization during wound repair.
30 wild type (WT) mice and 30 GMCSF- absence mice (GMCSF-KO) were obtained. They were received full thickness skin wound (0.8 cm x 0.8 cm) in each side of midline after deeply anesthesia. In the different post-injury time points, the wound sites were digitally photographed to calculate the percentage of wound closure by using computer image analyses software. The wound specimens were also obtained dynamically for immunohistological analysis of CD31 at wound site.
The analysis of the wound closure showed that wound healing in GMCSF-KO mice was delayed significantly comparing with that in WT mice from the day 3 post-wounding. At days 7 and 10 after wounding significantly more numbers of blood vessels were formed in the WT controls compared to the GMCSF-KO mice.
GMCSF-absence inhibits neovascularization during wound repair and leads to the delay of wound healing.

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Keywords

30 GMCSF- absence mice
 
30 wild type
 
computer image analyses software
 
day 3 post-wounding
 
different post-injury time points
 
full thickness skin wound
 
GMCSF-absence
 
GMCSF-absence inhibits neovascularization
 
GMCSF-KO
 
GMCSF-KO mice
 
immunohistological analysis
 
wound closure
 
wound healing
 
wound site
 
wound sites
 
wound specimens
 
WT
 
WT controls
 
WT mice