Article

A flow cytometric assay for analysis of natural-killer cell-mediated cytolysis of adenovirus-transformed cells.

Methods in molecular medicine 02/2007; 131:221-30. pp.221-30
Source: PubMed

ABSTRACT Natural-killer (NK) cells play an important role in recognizing and eliminating virally infected and transformed cells. To study this process, convenient assays for NK-cell function are required. Conventional NK-cell activity assays measure the release of 51Cr from prelabeled target cells following membrane disruption. This chapter describes nonradiometric assays for NK-cell killing of adenovirus-transformed human cells that can be applied to multiple cell samples using flow cytometry.

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Keywords

adenovirus-transformed human cells
 
flow cytometry
 
Natural-killer
 
nonradiometric assays
 
prelabeled target cells