High-throughput analysis of spatio-temporal dynamics in Dictyostelium.

Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
Genome biology (Impact Factor: 10.47). 02/2007; 8(7):R144. DOI: 10.1186/gb-2007-8-7-r144
Source: PubMed

ABSTRACT We demonstrate a time-lapse video approach that allows rapid examination of the spatio-temporal dynamics of Dictyostelium cell populations. Quantitative information was gathered by sampling life histories of more than 2,000 mutant clones from a large mutagenesis collection. Approximately 4% of the clonal lines showed a mutant phenotype at one stage. Many of these could be ordered by clustering into functional groups. The dataset allows one to search and retrieve movies on a gene-by-gene and phenotype-by-phenotype basis.

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