Evidence of Temporary Airway Epithelial Repopulation and Rare Clonal Formation by BM-derived Cells Following Naphthalene Injury in Mice

Children's Hospital Oakland Research Institute, Oakland, California 94609, USA.
The Anatomical Record Advances in Integrative Anatomy and Evolutionary Biology (Impact Factor: 1.54). 09/2007; 290(9):1033-45. DOI: 10.1002/ar.20574
Source: PubMed


The goal of the study was to investigate participation of bone marrow (BM) cells in the process of airway epithelial restoration after naphthalene-induced injury. We transplanted sex-mismatched green fluorescent protein (GFP) -tagged BM-derived cultured plastic-adherent mesenchymal stem cells into 5Gy-irradiated C57BL/6 recipients. After 1 month of recovery, experimental animals were subjected to 250 mg/kg naphthalene IP. Animals were killed at 2-30 days after naphthalene. By immunofluorescence, immunohistochemistry, and by in situ hybridization for the Y-chromosome, we observed patches of donor-derived cells in the large and small conducting airways, mostly at 2-6 days after injury. GFP(+) cells in the epithelium of airways were positive for pancytokeratin and some other epithelial markers. Although rare, GFP(+) cells formed clear isolated patches of the bronchial epithelium, consistent with clonal formation; as some cells were also positive for proliferating cell nuclear antigen, a marker of proliferating cells. After day 12, only occasional GFP(+) cells were present in the epithelium. These data confirm that bone marrow-derived cultured mesenchymal cells can participate in the recovery of the injured airway epithelium after naphthalene-induced injury with minimal long-term engraftment.

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Available from: Viacheslav Mikhailovich Mikhailov, Oct 14, 2014
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    • "In 2001, Krause and colleagues [26] published compelling evidence on the fact that a rare population of single BM-derived cells was able to repopulate the hematopoietic system and generate nonhematopoietic cell types in multiple tissues including epithelial cells of the liver, lung, skin, and gastrointestinal tract. Subsequently, several studies in murine models have demonstrated the ability of marrow-derived hematopoietic stem/progenitor cells (HSPCs) to home to the lung and engraft as airway and respiratory epithelial cells [30–33]. Others have shown that blood-borne stem cells may contribute to lung tissue in human recipients of bone marrow or lung transplantation [34–36]. "
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    The Scientific World Journal 01/2014; 2014:859817. DOI:10.1155/2014/859817 · 1.73 Impact Factor
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    • "The number of publications in The Anatomical Record that are related to regenerative medicine exploded in the 21st century. One paper, which reported temporary airway epithelial repopulation and rare clonal formation by mesenchymal stem cells after injury to the lung of mice (Serikov et al., 2007), provided the cover illustration for the Journal (Volume 290, 2007). Nearly 20 papers reported results for the cardiovascular system, including the heart (Eisenberg and Eisenberg, 2004; Rosen et al., 2004; Perez-Pomares et al., 2006). "

    The Anatomical Record Advances in Integrative Anatomy and Evolutionary Biology 01/2014; 297(1). DOI:10.1002/ar.22811 · 1.54 Impact Factor
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    • "In vivo experiments have shown the participation of BMSC in liver regeneration after CCl 4 injections [28]. There are also some data on the involvement of BMSC in lung airway epithelium renewal after chemical or bacterial damage [29] [30]. Nowadays the experiments on BMSC contribution to lung regeneration are being subjected to critical analysis [31]. "
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