Article

Replication of an RNA ligase ribozyme under alternating temperature condition.

Department of Biomaterial Control, Dong-Eui University, Busan 614-714, Republic of Korea.
FEBS Letters (impact factor: 3.54). 09/2007; 581(21):4065-72. DOI:10.1016/j.febslet.2007.07.048 pp.4065-72
Source: PubMed

ABSTRACT Self-replication process of the RNA ligase ribozyme molecules was investigated by using the modified RNA ligase ribozyme under alternating temperature condition that enhances turnover rate of the RNA ligation reaction. In our experiment, the RNA ligase ribozyme system mainly undergoes a cross-catalytic replication process, in which two ribozymes catalyze each other's synthesis from a total of four RNA substrates under alternating temperature condition, resulting in time-dependent accumulation of additional copies of the starting ribozymes in a reaction mixture. The present study demonstrates that cross-catalytic replication in nucleic acids system can be efficiently devised under the alternating temperature condition.

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Keywords

alternating temperature condition
 
cross-catalytic replication
 
cross-catalytic replication process
 
enhances turnover rate
 
modified RNA ligase ribozyme
 
nucleic acids system
 
other's synthesis
 
RNA ligase ribozyme molecules
 
RNA ligase ribozyme system
 
Self-replication process
 
two ribozymes catalyze