Random amplified polymorphic DNA typing of nosocomial Candida albicans isolates.

Department of Microbiology, Sur Hospital, Ministry of Health, Oman, PO Box 346, Postal Code 411, Sur, Oman.
Saudi medical journal (Impact Factor: 0.59). 09/2007; 28(8):1296-8.
Source: PubMed
Download full-text


Available from: Harish Gugnani, Sep 25, 2015
25 Reads
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The patterns of genetic variation of samples of Candida spp. isolated from patients infected with human immunodeficiency virus in Vitória, state of Espírito Santo, Brazil, were examined. Thirty-seven strains were isolated from different anatomical sites obtained from different infection episodes of 11 patients infected with the human immunodeficiency virus (HIV). These samples were subjected to randomly amplified polymorphic DNA (RAPD) analysis using 9 different primers. Reproducible and complex DNA banding patterns were obtained. The experiments indicated evidence of dynamic process of yeast colonization in HIV-infected patients, and also that certain primers are efficient in the identification of species of the Candida genus. Thus, we conclude that RAPD analysis may be useful in providing genotypic characters for Candida species typing in epidemiological investigations, and also for the rapid identification of pathogenic fungi.
    Memórias do Instituto Oswaldo Cruz 04/2004; 99(2):147-52. DOI:10.1590/S0074-02762004000200006 · 1.59 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: During the 1980s, the frequency of nosocomial candidiasis increased dramatically. This trend has continued into the 1990s, and Candida species remain a major cause of nosocomial infections. Although Candida albicans remains the most frequent cause of fungemia and hematogenously disseminated candidiasis, a number of reports have documented infections caused by other Candida species: C. tropicalis, C. glabrata, C. parapsilosis, C. krusei, and C. lusitaniae. Many of these infections arise from an endogenous source, and their frequency is influenced by the patient population, the various treatment regimens, and the antibiotics or other supportive care measures employed at specific institutions. Additional infections may be accounted for by exogenous acquisition via the hands of health care workers, contaminated infusates and biomaterials, and the inanimate environment. Ongoing investigation should help improve our understanding of the epidemiology of candidiasis and facilitate the development of rational preventive measures.
    Clinical Infectious Diseases 06/1996; 22 Suppl 2(Suppl 2):S89-94. DOI:10.1093/clinids/22.Supplement_2.S89 · 8.89 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Early identification of Candida isolates to the species level is necessary for effective antifungal therapy, and can also facilitate control of hospital infections. Phenotype-based methods for identifying Candida species are often difficult and time-consuming. Molecular biological techniques provide a useful alternative approach. In the present study, the ITS1-5.8S-ITS2 regions of fungal rRNA genes were amplified with universal primers in 20 standard strains. Digestion of the PCR products with one restriction enzyme, MspI, allowed discrimination of medically important Candida species, including C. albicans, C. glabrata, C. parapsilosis, C. tropicalis, C. krusei, and C. guilliermondii. Using this method, we successfully identified 137 clinical isolates of Candida. Among them, C. albicans was identified as the most common species, followed by C. parapsilosis, C. tropicalis, C. glabrata, C. krusei, and C. guilliermondii. This method is a simple, rapid, and cost-effective method for differentiation between species that is applicable in clinical laboratories.
    Nippon Ishinkin Gakkai Zasshi 02/2006; 47(3):225-9. DOI:10.3314/jjmm.47.225
Show more