Article

Pentamer is the minimum structure for oligomannosylpeptoids to bind to concanavalin A.

Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 J2-10, Nagatsutacho, Midoriku, Yokohama 226-8501, Japan.
Bioorganic & Medicinal Chemistry Letters (impact factor: 2.55). 10/2007; 17(18):5274-8. DOI:10.1016/j.bmcl.2006.12.075 pp.5274-8
Source: PubMed

ABSTRACT Enzyme-linked lectin assay (ELLA) was performed for oligomannosylpeptoids, which were immobilized on microtiter plates through a streptavidin-biotin interaction. The other immobilization methods, a hydrophobic adsorption and a covalent attachment, were found inapplicable to the oligomannosylpeptoids. Penta- and hexamannosylpeptoids with a shorter or longer spacer were found to be significantly recognized by concanavalinA (ConA), while the smaller peptoids showed no bindings. A proportional relationship between the amount of bound ConA and the peptoid density on the microtiter plate was observed, indicating the absence of both cluster and overdense effects that would assist or inhibit the binding increasingly with the ligand density.

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    Larissa Ishikawa, Thaís França, Fernanda Chiuso-Minicucci, Sofia Zorzella-Pezavento, Nelson Marra, Paulo Pereira, Célio Silva, Alexandrina Sartori
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    [show abstract] [hide abstract]
    ABSTRACT: Protein-calorie malnutrition (PCM) is the most common type of malnutrition. PCM leads to immunodeficiency and consequent increased susceptibility to infectious agents. In addition, responses to prophylactic vaccines depend on nutritional status. This study aims to evaluate the ability of undernourished mice to mount an immune response to a genetic vaccine (pVAXhsp65) against tuberculosis, containing the gene coding for the heat shock protein 65 from mycobacteria. Young adult female BALB/c mice were fed ad libitum or with 80% of the amount of food consumed by a normal diet group. We initially characterized a mice model of dietary restriction by determining body and spleen weights, hematological parameters and histopathological changes in lymphoid organs. The ability of splenic cells to produce IFN-gamma and IL-4 upon in vitro stimulation with LPS or S. aureus and the serum titer of specific IgG1 and IgG2a anti-hsp65 antibodies after intramuscular immunization with pVAXhsp65 was then tested. Dietary restriction significantly decreased body and spleen weights and also the total lymphocyte count in blood. This restriction also determined a striking atrophy in lymphoid organs as spleen, thymus and lymphoid tissue associated with the small intestine. Specific antibodies were not detected in mice submitted to dietary restriction whereas the well nourished animals produced significant levels of both, IgG1 and IgG2a anti-hsp65. 20% restriction in food intake deeply compromised humoral immunity induced by a genetic vaccine, alerting, therefore, for the relevance of the nutritional condition in vaccination programs based on these kinds of constructs.
    Genetic Vaccines and Therapy 02/2009; 7:11. · 2.10 Impact Factor
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Keywords

covalent attachment
 
ELLA
 
Enzyme-linked lectin assay
 
hexamannosylpeptoids
 
immobilization methods
 
immobilized
 
ligand density
 
microtiter plate
 
microtiter plates
 
oligomannosylpeptoids
 
overdense effects
 
peptoid density
 
proportional relationship
 
smaller peptoids
 
streptavidin-biotin interaction
 

Hideya Yuasa