Selection using the α1-integrin (CD49a) enhances the multipotentiality of the mesenchymal stem cell population from heterogeneous bone marrow stromal cells

Laboratory of Stem Cells and Tissue Repair, Institute of Molecular and Cell Biology, Singapore, Singapore.
Journal of Molecular Histology (Impact Factor: 1.82). 11/2007; 38(5):449-58. DOI: 10.1007/s10735-007-9128-z
Source: PubMed


Bone marrow-derived mesenchymal stem cells consist of a developmentally heterogeneous population of cells obtained from colony forming progenitors. As these colonies express the alpha-1 integrin (CD49a), here we single-cell FACS sorted CD49a+ cells from bone marrow in order to create clones and then compared their colony forming efficiency and multilineage differentiation capacity to the unsorted cells. Following selection, 40% of the sorted CD49a+ cells formed colonies, whereas parental cells failed to form colonies following limited dilution plating at 1 cell/well. Following ex vivo expansion, clones shared a similar morphology to the parental cell line, and also demonstrated enhanced proliferation. Further analysis by flow cytometry using a panel of multilineage markers demonstrated that the CD49a+ clones had enhanced expression of CD90 and CD105 compared to unsorted cells. Culturing cells in adipogenic, osteogenic or chondrogenic medium for 7, 10 and 15 days respectively and then analysing them by quantitative PCR demonstrated that CD49a+ clones readily underwent multlineage differentiation into fat, bone and cartilage compared to unsorted cells. These results thus support the use of CD49a selection for the enrichment of mesenchymal stem cells, and describes a strategy for selecting the most multipotential cells from a heterogeneous pool of bone marrow mononuclear stem cells.

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    • "CD105 (human endoglin) is a homodimeric integral membrane glycoprotein, belonging to the transforming growth factor-b (TGF-b) receptor system, highly expressed in mesenchymal stem cells [57]. A higher expression of CD105 in cells has been correlated with higher growth kinetics and differentiation potential [58] [59]. However, adherent cells isolated from SAT and DAT showed comparable proliferation capacity and adipogenic potential. "
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    Cytotherapy 05/2015; 17(8). DOI:10.1016/j.jcyt.2015.04.004 · 3.29 Impact Factor
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    • "The absence of CD31 is a typical feature of MSCs.22 CD45 (protein tyrosine phosphatase receptor type C or leukocyte common antigen) can be found on the surface of differentiated haematopoietic cells, and it plays an important role in signal transduction to T- and B-cell receptors; therefore, it has been used as negative selective marker.23,24 CD49a, or alpha-1 integrin, has been used to isolate MSCs from bone marrow.25,26 CD49b, or alpha-2 integrin, participates in collagen I binding and might play a role in the survival of MSCs on collagen I.8 CD49d is the alpha-4 integrin subunit that is present in human MSCs under certain culture conditions.27 "
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    International Journal of Oral Science 05/2014; 6(3). DOI:10.1038/ijos.2014.23 · 2.53 Impact Factor
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    • "3G5 has not been tested as a cell surface marker for PMSCs and the expression patterns of STRO-1 and 3G5 in the placenta are not known. Another marker which has been used to enrich the CFU-F population is CD49a [24]. Integrin alpha 1 (CD49a/VLA-1), which is the receptor for laminin and collagen, was also investigated. "
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