Article

E. coli K-12 asparaginase-based asparagine biosensor for leukemia.

Biosensor Development Laboratory, Department of Biotechnology, Punjabi University, Patiala, India.
Artificial Cells Blood Substitutes and Biotechnology (impact factor: 0.98). 02/2007; 35(4):449-56. DOI:10.1080/10731190701460358 pp.449-56
Source: PubMed

ABSTRACT The present work aims at the development of a novel, diagnostic biosensor for monitoring asparagine levels in leukemia. Various immobilization strategies have been applied to improve the stability of the biocomponent (asparaginase). Response time studies have been carried out for different immobilization methods. Phenol Red indicator has been coimmobilized with asparaginase and color visualization approach has been optimized for various asparagine ranges. The detection limit of asparagine achieved with nitrocellulose membrane is 10(-1) M, with silicon gel is 10(-10)-10(-1) M, and with calcium alginate beads is 10(-9)-10(-1) M. Furthermore, the calcium alginate bead system of immobilization has been applied for the asparagine range detection in normal and leukemia serum samples.

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Keywords

asparagine
 
asparagine range detection
 
biocomponent
 
color visualization approach
 
different immobilization methods
 
leukemia
 
leukemia serum samples
 
monitoring asparagine levels
 
nitrocellulose membrane
 
Phenol Red indicator
 
Response time studies
 
silicon gel
 
various asparagine ranges
 

Neelam Verma