Article

In vitro anti-Helicobacter pylori activity of diallyl sulphides and protocatechuic acid.

Department of Nutritional Science, Chung Shan Medical University, Taichung City, Taiwan, ROC.
Phytotherapy Research (impact factor: 2.09). 01/2008; 22(1):53-7. DOI:10.1002/ptr.2259 pp.53-7
Source: PubMed

ABSTRACT The in vitro inhibitory effects of diallyl disulphide (DADS), diallyl trisulphide (DAT), roselle calyx extract and protocatechuic acid (PA) on the growth of Helicobacter pylori (15 susceptible, 11 clarithromycin-resistant and 9 metronidazole-resistant strains) were studied. The inhibition zone was determined after each agent had been heated at 25, 60, 100 degrees C for 60 min. The minimal inhibitory concentration (MIC) of each agent was determined by the tube dilution assay. The results showed that heat treatment did not affect the anti-H. pylori activity of DADS, DAT, roselle calyx extract and PA, and the MIC values of these agents against test H. pylori strains were in the range 8-64 mg/L. The time-kill study assay for DAT and PA at 1x MIC was monitored in Muller Hinton broth supplemented with 10% horse blood or mice stomach homogenate. Both DAT and PA inhibited the growth of all test H. pylori in broth and mice stomach homogenate (p < 0.05); however, the inhibitory effects of these two agents were less in mice stomach homogenate than in broth (p < 0.05). DAT at 4, 6, 8, 10, 12, 14 mg/L and PA at 8, 16, 24, 32, 40, 48 mg/L were used for urease activity assay. These two agents significantly reduced urease activity of test H. pylori strains (p < 0.05), in which DAT and PA at 1x MIC reduced the urease activity of H. pylori to 70% and 40%, respectively. These agents, based on their lower MIC values and heat tolerance, might be useful in the prevention or therapy of H. pylori.

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    Article: Production of Protocatechuic Acid in Bacillus Thuringiensis ATCC33679.
    Kimtrele M Williams, William E Martin, Justin Smith, Baraka S Williams, Bianca L Garner
    [show abstract] [hide abstract]
    ABSTRACT: Protocatechuic acid, or 3,4-dihydroxybenzoic acid, is produced by both soil and marine bacteria in the free form and as the iron binding component of the siderophore petrobactin. The soil bacterium, Bacillus thuringiensis kurstaki ATCC 33679, contains the asb operon, but does not produce petrobactin. Iron restriction resulted in diminished B. thuringiensis kurstaki ATCC 33679 growth and the production of catechol(s). The gene product responsible for protocatechuic acid (asbF) and its receptor (fatB) were expressed during stationary phase growth. Gene expression varied with growth temperature, with optimum levels occurring well below the Bacillus anthracis virulence temperature of 37 °C. Regulation of protocatechuic acid suggests a possible role for this compound during soil growth cycles.
    International Journal of Molecular Sciences 01/2012; 13(3):3765-72. · 2.60 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

9 metronidazole-resistant strains
 
H. pylori
 
heat tolerance
 
Helicobacter pylori
 
inhibition zone
 
inhibitory effects
 
lower MIC values
 
MIC values
 
mice stomach homogenate
 
Muller Hinton broth supplemented
 
pylori activity
 
roselle calyx
 
test H. pylori
 
test H. pylori strains
 
time-kill study assay
 
tube dilution assay
 
two agents
 
urease activity
 
urease activity assay
 
vitro inhibitory effects
 

Wen-Hu Liu