Effects of maternal strain on ethanol responses in reciprocal F1 C57BL/6J and DBA/2J hybrid mice.

Department of Behavioral Neuroscience and Portland Alcohol Research Center, Oregon Health & Science University, Portland, OR, USA.
Genes Brain and Behavior (Impact Factor: 3.51). 05/2008; 7(3):276-87. DOI: 10.1111/j.1601-183X.2007.00349.x
Source: PubMed

ABSTRACT Variations in maternal behavior, either occurring naturally or in response to experimental manipulations, have been shown to exert long-lasting consequences on offspring behavior and physiology. Despite previous research examining the effects of developmental manipulations on drug-related phenotypes, few studies have specifically investigated the influence of strain-based differences in maternal behavior on drug responses in mice. The current experiments used reciprocal F1 hybrids of two inbred mouse strains (i.e. DBA/2J and C57BL/6J) that differ in both ethanol (EtOH) responses and maternal behavior to assess the effects of maternal environment on EtOH-related phenotypes. Male and female DBA/2J and C57BL/6J mice and their reciprocal F1 hybrids reared by either DBA/2J or C57BL/6J dams were tested in adulthood for EtOH intake (choice, forced), EtOH-induced hypothermia, EtOH-induced activity and EtOH-induced conditioned place preference (CPP). C57BL/6J and DBA/2J mice showed differences on all EtOH responses. Consistent with previous reports that maternal strain can influence EtOH intake, F1 hybrids reared by C57BL/6J dams consumed more EtOH during forced exposure than did F1 hybrids reared by DBA/2J dams. Maternal strain also influenced EtOH-induced hypothermic responses in F1 hybrids, producing differences in hybrid mice that paralleled those of the inbred strains. In contrast, maternal strain did not influence EtOH-induced activity or CPP in hybrid mice. The current findings indicate that maternal environment may contribute to variance in EtOH-induced hypothermia and EtOH intake, although effects on EtOH intake appear to be dependent upon the type of EtOH exposure.

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Available from: Kara I Gabriel, Nov 11, 2014
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    • "Alcohol preference test: two-bottle free choice A TBC paradigm with escalating ethanol concentrations was used (Thiele et al. 1998; Li et al. 2005; Gabriel and Cunningham 2008) to examine ethanol preference. Mice (54 C57Bl/6J, "
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    ABSTRACT: Exposure to inescapable stressors increases both the rewarding properties and self-administration of cocaine through the signaling of the kappa-opioid receptor (KOR), but the effect of this signaling on other reinforcing agents remains unclear. The objective of this study is to test the hypothesis that signaling of the KOR mediates the forced swim stress (FSS)-induced potentiation of ethanol reward and self-administration. Male C57Bl/6J mice were tested in a biased ethanol-conditioned place preference (CPP) procedure, and both C57Bl/6J and prodynorphin gene-disrupted (Dyn -/-) mice were used in two-bottle free choice (TBC) assays, with or without exposure to FSS. To determine the role of the KOR in the resulting behaviors, the KOR agonist U50,488 (10 mg/kg) and antagonist nor-binaltorphimine (nor-BNI, 10 mg/kg) were administered prior to parallel testing. C57Bl/6J mice exposed to repeated FSS 5 min prior to daily place conditioning with ethanol (0.8 g/kg) demonstrated a 4.4-fold potentiation of ethanol-CPP compared to unstressed mice that was prevented by nor-BNI pretreatment. Likewise, pretreatment with U50,488 90 min prior to daily ethanol place conditioning resulted in a 2.8-fold potentiation of ethanol-CPP. In the TBC assay, exposure to FSS significantly increased the consumption of 10% (v/v) ethanol by 19.3% in a nor-BNI-sensitive manner. Notably, Dyn -/- mice consumed a similar volume of ethanol as wild-type littermates and C57Bl/6J mice, but did not demonstrate significant stress-induced increases in consumption. These data demonstrated a stress-induced potentiation of the rewarding effects and self-administration of ethanol mediated by KOR signaling.
    Psychopharmacology 06/2010; 210(2):199-209. DOI:10.1007/s00213-010-1844-5 · 3.99 Impact Factor
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    • "Behavioral changes have also been observed in hybrids, such as the temperament differences commonly reported between hinnies (donkey mother and horse father) and mules (horse mother and donkey father) (Gray, 1972). In the laboratory, the reciprocal breeding of various inbred and outbred rodent strains and species has been extremely useful in screening for parent-of-origin effects on behavioral and physiological phenotypes such as emotional reactivity (Calatayud & Belzung, 2001; Carola, Frazzetto, & Gross, 2006; Roy, Merali, Poulter, & Anisman, 2007), maternal care (Calatayud, Coubard, & Belzung, 2004; Carola et al., 2008; Shoji & Kato, 2009), infanticide (Perrigo et al., 1993), aggression (Carlier, Roubertoux, & Pastoret, 1991; Platt & Maxson, 1989), sex (McGill & Manning, 1976), forced ethanol intake (Gabriel & Cunningham, 2008), calcium taste preference (Tordoff, Reed, & Shao, 2008), activity (Dohm, Richardson , & Garland, 1994; Massett & Berk, 2005; Price & Loomis, 1973), cerebellar development (Cooper, Benno, Hahn, & Hewitt, 1991), peripheral nerve conductivity (Hegmann & White, 1973), central estrogen receptor a distribution (Kramer, Carr, Schmidt, & Cushing, 2006), and puberty onset (Zhou et al., 2007). Though it is clear from these studies that parent-oforigin effects exist, it is extremely challenging to delineate which mechanisms may be partially or fully responsible for each of these phenotypes. "
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    • "There has been little investigation of EtOH drinking levels in hybrid mice in which they have been compared to the progenitors used to derive them. However, F1 crosses involving, B6, D2, A/J, BALB/cJ, and CD-1 have shown largely intermediate EtOH consumption amounts in 24-h choice procedures, and in no case was over-dominance seen (Goodrick 1978; Short et al. 2006; Gabriel and Cunningham 2008). The QTL we have identified would be expected to be unique to populations carrying the same alleles as B6 and FVB mice, making intralocus interaction of the two allele types possible. "
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    ABSTRACT: Excessive alcohol (ethanol) consumption is the hallmark of alcohol use disorders. The F1 hybrid cross between the C57BL/6J (B6) and FVB/NJ (FVB) inbred mouse strains consumes more ethanol than either progenitor strain. The purpose of this study was to utilize ethanol-drinking data and genetic information to map genes that result in overdominant (or heterotic) ethanol drinking. About 600 B6 x FVB F2 mice, half of each sex, were tested for ethanol intake and preference in a 24-h, two-bottle water versus ethanol choice procedure, with ascending ethanol concentrations. They were then tested for ethanol intake in a Drinking in the Dark (DID) procedure, first when there was no water choice and then when ethanol was offered versus water. DNA samples were obtained and genome-wide QTL analyses were performed to search for single QTLs (both additive and dominance effects) and interactions between pairs of QTLs, or epistasis. On average, F2 mice consumed excessive amounts of ethanol in the 24-h choice procedure, consistent with high levels of consumption seen in the F1 cross. Consumption in the DID procedure was similar or higher than amounts reported previously for the B6 progenitor. QTLs resulting in heightened consumption in heterozygous compared to homozygous animals were found on Chrs 11, 15, and 16 for 24-h choice 30% ethanol consumption, and on Chr 11 for DID. No evidence was found for epistasis between any pair of significant or suggestive QTLs. This indicates that the hybrid overdominance is due to intralocus interactions at the level of individual QTL.
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