A randomized and controlled comparison of the wash-resistances and insecticidal efficacies of four types of deltamethrin-treated nets, over a 6-month period of domestic use with washing every 2 weeks, in a rural area of Iran

Disease Control and Vector Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
Pathogens and Global Health (Impact Factor: 1.66). 10/2007; 101(6):519-28. DOI: 10.1179/136485907X193815
Source: PubMed


In a randomized, prospective, 6-month-long field study in a rural area of Iran, the wash resistances of 200 nets (40 PermaNet, 40 Yorkool and 40 A-Z nets), that their manufacturers claimed be long-lasting insecticidal nets (LLIN), were compared with those of 40 nets conventionally treated with deltamethrin (using K-O Tab tablets). All the nets were kept in routine domestic use and subjected to standardized hand-washing at 2-week intervals. Wild-caught or laboratory-reared Anopheles stephensi were used for the bio-assays of insecticidal activity. The regular washing and domestic use led to reductions in the insecticidal activities of all the treated nets after 6 months. Although the PermaNet nets showed the smallest reduction, they were not significantly better than the conventionally treated nets, which still showed acceptable insecticidal activity after 6 months. The PermaNet and A-Z nets both performed significantly better than the Yorkool nets, which were slightly but not significantly worse than the conventionally treated nets. In questionnaire-based interviews, the local householders were found to wash their own (non-study) nets at median and mean frequencies of every 2 and 2.1 weeks, respectively. In conclusion, the PermaNet nets showed better wash resistance than any of the other commercial nets, and were the only commercial nets tested that truly appeared to be LLIN. There still appears to be scope, however, for the impregnation, and thus the wash-resistance, of even the PermaNet nets to be improved.

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    • "), in field conditions, in experimental huts (Asidi et al. 2005; Graham et al. 2005; Kayedi et al. 2007b; Malima et al. 2008), and at the community level (Alonso et al. 1993; Binka et al. 1996; Lengeler 2004; Nevill et al. 1996). Unfortunately, at that community level, most of the conventional bednets are still untreated mainly due to insecticide unavailability and to the distance between houses and treatment sites (Okrah et al. 2002). "
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    ABSTRACT: Insecticide-treated bednets play a cornerstone role in the efforts to control malaria. Bednets entomological efficacy is the determinant factor of their use to control malaria. In this study, we compared under laboratory conditions, the efficacy of two long-lasting nets (PermaNet® versus Interceptor®) and two treatments kits K-O TAB® (deltamethrin) versus Fendona 6SC® (alpha-cypermethrin) against Anopheles gambiae s.l. malaria vectors. The efficacy of washed and unwashed bednets was assessed by contact bioassays using World Health Organization (WHO) cones. Three to five-days-old mosquitoes were exposed to the netting for 3 min; the median and 95 % knockdown time, the after 24 h mortality was recorded for each type of bednet. The mortality after 24 h was equivalent for the Fendona 6SC® treated bednets and the K-O TAB® treated bednets [79.4 % confidence limits (CL) (73.9-84.6) and 74 % CL (68.3-80.0), respectively]. However, the Fendona 6SC® treated bednets were superior in 50 % knockdown time to the K-O TAB® treated bednets [7.8 min, CL (6.5-9.0) and 15. 2 min, CL (14.0-16.4), respectively]. Washed Interceptor® and PermaNet® bednets showed similar efficacy in terms of 50 % knockdown times. Mortality after 24 h was similar from the fifth to the twentieth wash, but PermaNet® performed better than Interceptor® for the first four washes and for unwashed bednets. This study showed that Fendona 6SC® kit and the Interceptor® bednets have exhibited consistent comparable efficacy in the laboratory compared to the well known and in use K-O TAB® kit and PermaNet® bednets.
    Parasitology Research 01/2014; 113(3). DOI:10.1007/s00436-013-3742-3 · 2.10 Impact Factor
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    • "With the decrease of α long-lasting alpha-cypermethrin treated residue due to repeated washing, the entry index increased. The are several reports on the bioefficacy of bednet worldwide (Gimnig 2005, Kayedi et al. 2007, 2009, Gunasekaran and Vaidyanathan 2008, Oxborough et al. 2009). "
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    ABSTRACT: The Long-lasing insecticide impregnated nets (LLINs) is considered as an effective tools for malaria vector control. The aim of this study was to evaluate the residual efficacy of alpha-cypermethrin long lasting impregnated nets (LLIN-Interceptor(®)) against Anopheles stephensi using tunnel test. The wash-resistance of Interceptor(®) nets were assessed under laboratory conditions using tunnel test. Females of An. stephensi were released into the tunnel and then they were provided blood meals from guinea pigs. Bed nets were washed according to the standard procedure up to 20 times. The bioefficacy indicators such as inhibition of bloodmeal from experimental animal, knockdown, irritancy rate, survival rate, entry index and mortality were calculated. It induced 90-100% mortalities in the population of An. stephensi up to 15 washes. The KT50 values reduced from 73.47 to 26.30 minutes in unwashed in comparison to one washed, respectively. The mean of mortality rate of blood-feeding inhibition and entry indexes was reached to 91.6%±2.8, 87.0±3.4 and 24.9±2.8 respectively after 20 washing. This net could provide a good personal protection against malaria vectors and could induce relatively high mortality, inhibit the blood-feeding as well as reduce the entry rates of female mosquitoes even after several washes.
    Journal of Arthropod-Borne Diseases 04/2013; 7(1):31-45. · 0.68 Impact Factor
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    • "In other studies (Graham et al, 2005; Sreehari et al, 2007a) the quantity of detergent ranged from 0.6 to 1.0 g/liter. Kayedi et al (2007a) speculated various washing conditions and the use of different detergents and soaps in the wash resistance tests with LLINs in various laboratories and field conditions contributed to different results with the same product causing difficulty in comparing results ; 3) relatively longer intervals between successive washes: 12 days in the present study compared to 2-6 days in other studies (Graham et al, 2005; Sreehari et al, 2007a) allowing higher diffusion of deltamethrin from inside to the outer surfaces of the fibers thereby making it more susceptible to removal with the next wash (Kayedi et al, 2007b). "
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    ABSTRACT: Wash resistance and field bioefficacy of PermaNet® 2.0 nets, long lasting insecticidal nets, against mosquitoes were evaluated in Assam, northeastern India. After repeated hand washings at 12-day intervals a decline in the mosquito killing ability of PermaNet nets was noted (trend χ2 38.9, p
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