Long- and short-time immunological memory in different strains of mice given nasally an adjuvant-combined nasal influenza vaccine

Department of Applied Biochemistry, School or Engineering, Tokai University, Kitakaname 1117, Hiratsuka-shi, Kanagawa 259-1292, Japan.
Vaccine (Impact Factor: 3.62). 10/2007; 25(39-40):6975-80. DOI: 10.1016/j.vaccine.2007.06.060
Source: PubMed


Immunological memory induced by nasal immunization with adjuvant-combined influenza vaccine was analyzed in different ages and strains of mice. The memory activities were assessed by secondary nasal-wash IgA and serum IgG antibody (Ab) responses and protection against challenge infection with a lethal dose of influenza virus. Mice were primed with 0.1 microg of vaccine and boosted with 0.1 or 1.0 microg vaccine 1 (short-term memory)- or 17 (long-term memory)-months later. Influenza-specific short-term memory responses in young adult BALB/c mice (2-month-old) were significantly higher than those of long-term memory activities in mice boosted at 19 months of age. However, those influenza-specific long-term memory responses provided protective immunity against influenza virus challenge and were higher than short-term memory in aged mice primed at 18-month-old and boosted 1 month later. These results show that the age at which initial nasal immunization is given is critically important in order to induce protective immunity in aged mice. Similar findings were noted in the C3H mouse strain; however, C57BL/6 mice failed to induce influenza-specific immune responses in both young adult and aged mice. These results indicate that low doses of cholera toxin B subunit (supplemented with 0.2% of hole toxin) combined nasal vaccine may required further improvement in order to provide protective immunity in human use.

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    • "In addition to eliciting serum IgG, vaccines administered via the mucosal route can also elicit mucosal IgA in the respiratory tract. Mucosal antibodies can neutralize influenza virus at the site of entry, thus preventing infection [7] [8]. Gibbs et al. [9] underlined the importance of CD4 + Th-cells to generate and to maintain protective B-cell and CD8 + T-cell immunity to protect against highly virulent pathogens, such as influenza. "
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    Vaccine 01/2014; 32(20). DOI:10.1016/j.vaccine.2013.12.043 · 3.62 Impact Factor
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    • "More recently, it was reported that Swine-origin 2009A (H1N1) influenza virus isolated from humans can infect mice with a high infectivity (Maines et al., 2009). Currently, the murine model is widely employed as a useful model for the investigation of aging, as well as immune response to influenza virus infection (Effros et al., 1983; Po et al., 2002; Asanuma et al., 2007). "
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