Article
Application of fractal analysis to neuronal dendritic arborisation patterns of the monkey dentate nucleus.
Department of Biophysics, School of Medicine, University of Belgrade, Belgrade, Serbia.
Neuroscience Letters (Impact Factor: 2.03). 10/2007; 425(1):237. DOI: 10.1016/j.neulet.2007.08.009 Source: PubMed

Article: Agerelated reduction of structural complexity in spleen hematopoietic tissue architecture in mice.
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ABSTRACT: The effects of aging on structural complexity in hematopoietic tissue are unknown. In this work, in a mouse experimental model, we report the agerelated reduction of spleen hematopoietic tissue (SHT) complexity. Spleen tissue was obtained from the total of 64 male Swiss albino mice divided into 8 age groups: newborns (0 days old), 10 days, 20 days, 30 days, 120 days, 210 days, 300 and 390 days old. SHT was stained using conventional hematoxylin/eosin, and DNAbinding toluidine blue dyes. Fractal dimension as an indicator of cellular complexity, and lacunarity as indicator of tissue heterogeneity were determined based on the binarized SHT micrographs. Results indicate that fractal dimension of mice spleen hematopoietic tissue decreases with age, while lacunarity increases. These changes/trends have been detected in SHT stained both with toluidine blue and conventional hematoxylin/eosin. Fractal dimension was negatively correlated with lacunarity. The detected reduction in complexity suggests that agerelated structural changes are present in mouse SHT both in general tissue architecture and progenitor cell DNA.Experimental gerontology 07/2013; · 3.34 Impact Factor  [Show abstract] [Hide abstract]
ABSTRACT: Recent studies have suggested that silver nanoparticles (AgNPs) may affect cell DNA structure in in vitro conditions. In this paper, we present the results indicating that AgNPs change nuclear complexity properties in isolated human epithelial buccal cells in a timedependent manner. Epithelial buccal cells were plated in special tissue culture chamber / slides and were kept at 37°C in an RPMI 1640 cell culture medium supplemented with Lglutamine. The cells were treated with colloidal silver nanoparticles suspended in RPMI 1640 medium at the concentration 15 mg L(1) . Digital micrographs of the cell nuclei in a sample of 30 cells were created at five different time steps: before the treatment (controls), immediately after the treatment, as well as 15 , 30 and 60 min after the treatment with AgNPs. For each nuclear structure, values of fractal dimension, lacunarity, circularity, as well as parameters of grey level cooccurrence matrix (GLCM) texture, were determined. The results indicate timedependent reduction of structural complexity in the cell nuclei after the contact with AgNPs. These findings further suggest that AgNPs, at concentrations present in today's overthecounter drug products, might have significant effects on the cell genetic material.Journal of Microscopy 10/2013; · 1.63 Impact Factor  [Show abstract] [Hide abstract]
ABSTRACT: Abstract: Fractal analysis has become a popular method in all branches of scientific investigations including biology and medicine. Although there is a growing interest in the application of fractal analysis in biological sciences, questions about the methodology of fractal analysis have partly restricted its wider and comprehensible application. It is a notable fact that fractal analysis is derived from fractal geometry, but there are some unresolved issues that need to be addressed. In this respect, we discuss several related underlying principles for fractal analysis and establish the meaningful relationship between fractal analysis and fractal geometry. Since some concepts in fractal analysis are determined descriptively and/or qualitatively, this paper provides their exact mathematical definitions or explanations. Another aim of this study is to show that nowadays fractal analysis is an independent mathematical and experimental method based on Mandelbrot’s fractal geometry, Euclidean traditional geometry and Richardson’s coastline method.Theoretical Biology Forum. 11/2013; 105 (2). 2012, 99118.
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