Article

A proteomic screen reveals novel Fas ligand interacting proteins within nervous system Schwann cells.

Department of Physiology, McGill University, 3655 Promenade Sir William Osler, Montréal, Québec, Canada H3G 1Y6.
FEBS Letters (impact factor: 3.54). 10/2007; 581(23):4455-62. DOI:10.1016/j.febslet.2007.08.025 pp.4455-62
Source: PubMed

ABSTRACT Fas ligand (FasL) binds Fas (CD95) to induce apoptosis or activate other signaling pathways. In addition, FasL transduces bidirectional or 'reverse signals'. The intracellular domain of FasL contains consensus sequences for phosphorylation and an extended proline rich region, which regulate its surface expression through undetermined mechanism(s). Here, we used a proteomics approach to identify novel FasL interacting proteins in Schwann cells to investigate signaling through and trafficking of this protein in the nervous system. We identified two novel FasL interacting proteins, sorting nexin 18 and adaptin beta, as well as two proteins previously identified as FasL interacting proteins in T cells, PACSIN2 and PACSIN3. These proteins are all associated with endocytosis and trafficking, highlighting the tight regulation of cell surface expression of FasL in the nervous system.

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  • Chapter: The PACSIN proteins and their role in membrane trafficking.
    Plomann M, Mörgelin M, Schael S
    01/2009: pages 39-48;
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    Article: Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening.
    Matthias Voss, Marcus Lettau, Ottmar Janssen
    [show abstract] [hide abstract]
    ABSTRACT: Fas ligand is a cytotoxic effector molecule of T and NK cells which is characterized by an intracellular N-terminal polyproline region that serves as a docking site for SH3 and WW domain proteins. Several previously described Fas ligand-interacting SH3 domain proteins turned out to be crucial for the regulation of storage, expression and function of the death factor. Recent observations, however, indicate that Fas ligand is also subject to posttranslational modifications including shedding and intramembrane proteolysis. This results in the generation of short intracellular fragments that might either be degraded or translocate to the nucleus to influence transcription. So far, protein-protein interactions that specifically regulate the fate of the intracellular fragments have not been identified. In order to further define the SH3 domain interactome of the intracellular region of Fas ligand, we now screened a human SH3 domain phage display library. In addition to known SH3 domains mediating binding to the Fas ligand proline-rich domain, we were able to identify a number of additional SH3 domains that might also associate with FasL. Potential functional implications of the new binding proteins for the death factor's biology are discussed. For Tec kinases and sorting nexins, the observed interactions were verified in cellular systems by pulldown experiments. We provide an extended list of putative Fas ligand interaction partners, confirming previously identified interactions, but also introducing several novel SH3 domain proteins that might be important regulators of Fas ligand function.
    BMC Immunology 10/2009; 10:53. · 2.53 Impact Factor
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Keywords

'reverse signals'
 
activate
 
adaptin beta
 
cell surface expression
 
consensus sequences
 
extended proline rich region
 
Fas ligand
 
FasL
 
FasL interacting proteins
 
FasL transduces bidirectional
 
induce apoptosis
 
nervous system
 
novel FasL interacting proteins
 
PACSIN2
 
proteomics approach
 
Schwann cells
 
sorting nexin 18
 
surface expression
 
T cells