Article

Transcriptional profiling of chickpea genes differentially regulated in response to high-salinity, cold and drought

RMIT University, School of Applied Sciences, Biotechnology and Environmental Biology, Building 223, Level 1, Plenty Road, Bundoora, Victoria 3083, Australia.
BMC Genomics (Impact Factor: 4.04). 02/2007; 8:303. DOI: 10.1186/1471-2164-8-303
Source: PubMed

ABSTRACT Cultivated chickpea (Cicer arietinum) has a narrow genetic base making it difficult for breeders to produce new elite cultivars with durable resistance to major biotic and abiotic stresses. As an alternative to genome mapping, microarrays have recently been applied in crop species to identify and assess the function of putative genes thought to be involved in plant abiotic stress and defence responses. In the present study, a cDNA microarray approach was taken in order to determine if the transcription of genes, from a set of previously identified putative stress-responsive genes from chickpea and its close relative Lathyrus sativus, were altered in chickpea by the three abiotic stresses; drought, cold and high-salinity. For this, chickpea genotypes known to be tolerant and susceptible to each abiotic stress were challenged and gene expression in the leaf, root and/or flower tissues was studied. The transcripts that were differentially expressed among stressed and unstressed plants in response to the particular stress were analysed in the context of tolerant/susceptible genotypes.
The transcriptional change of more than two fold was observed for 109, 210 and 386 genes after drought, cold and high-salinity treatments, respectively. Among these, two, 15 and 30 genes were consensually differentially expressed (DE) between tolerant and susceptible genotypes studied for drought, cold and high-salinity, respectively. The genes that were DE in tolerant and susceptible genotypes under abiotic stresses code for various functional and regulatory proteins. Significant differences in stress responses were observed within and between tolerant and susceptible genotypes highlighting the multiple gene control and complexity of abiotic stress response mechanism in chickpea.
The annotation of these genes suggests that they may have a role in abiotic stress response and are potential candidates for tolerance/susceptibility.

2 Followers
 · 
391 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Cold stress at reproductive phase in susceptible chickpea (Cicer arietinum L.) leads to pollen sterility induced flower abortion. The tolerant genotypes, on the other hand, produce viable pollen and set seed under cold stress. Genomic information on pollen development in cold-tolerant chickpea under cold stress is currently unavailable.
    BMC Research Notes 10/2014; 7(1):717. DOI:10.1186/1756-0500-7-717
  • [Show abstract] [Hide abstract]
    ABSTRACT: The effects of TiO2 nanoparticles (NPs) on physiologo-biochemical responses were studied in two chickpea (Cicer arietimun L.) genotypes differing in cold sensitivity (tolerant Sel11439 and sensitive ILC533) during cold stress (CS). The results showed that hydrogen peroxide and MDA contents and electrolyte leakage index (ELI) increased under CS conditions in both genotypes and that these damage indices were higher in ILC533 than in Sel11439 plants. In plants treated with TiO2NPs, a decreased H2O2 level was accompanied by a decrease in the MDA content and ELI compared to control plants, and these changes occurred more effectively in Sel11439 than in ILC533 plants. The antioxidant enzymes were more effective in cell protection against CS in Sel11439 plants compared to ILC533 plants, as well as in plants treated with TiO2NPs compared to control plants. The lipoxygenase activity was induced efficiently only in Sel11439 plants treated with TiO2NPs during CS, probably indicating its role in stress response (which was confirmed by measuring allen oxide synthase activity). TiO2NPs caused stability of chlorophyll and carotenoid contents during CS. Results suggest that TiO2NPs confer an increased tolerance of chickpea plants to CS, decreasing the level of injuries and increasing the capacity of defense systems.
    Russian Journal of Plant Physiology 11/2014; 61(6). DOI:10.1134/S1021443714050124 · 0.76 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Identification of defense response genes in the host is one of the most essential steps in understanding disease resistance mechanisms in plants. In this study, suppression subtractive hybridization (SSH) library was constructed to study the genes involved in response to fusarium wilt disease in banana. Here cDNAs from a tolerant genotype Musa acuminata spp. burmannicoides ‘Calcutta-4’ infected by Fusarium oxysporum f.sp., cubense were used as tester and cDNAs from uninfected ‘Calcutta-4’ as driver population. After hybridization and cloning, EST library of 83 non-redundant clones were obtained. Based on sequence analysis and homology search in NCBI database the clones were assigned to different functional categories. The expression pattern of selected eight defense related genes namely peroxidase, glutaredoxin, polyphenol oxidase, glutamate synthase, S-adenosyl methionine synthetase, 14-3-3, heat shock protein, mannose binding lectin were analyzed through real-time PCR in contrasting genotypes. It was observed that the expression of these genes during initial progression of disease was found to be higher in tolerant genotype ‘Calcutta-4’ than in susceptible genotype ‘Kadali’.
    Physiological and Molecular Plant Pathology 07/2013; 83:1-7. DOI:10.1016/j.pmpp.2013.02.003 · 1.99 Impact Factor

Full-text (4 Sources)

Download
31 Downloads
Available from
May 20, 2014

Similar Publications