Transcriptome analysis of Yersinia pestis in human plasma: an approach for discovering bacterial genes involved in septicaemic plague

Yersinia Research Unit, Institut Pasteur, 28 rue du Dr. Roux, F-75724 Paris cedex 15, France.
Microbiology (Impact Factor: 2.56). 10/2007; 153(Pt 9):3112-24. DOI: 10.1099/mic.0.2007/006213-0
Source: PubMed

ABSTRACT Yersinia pestis is the aetiologic agent of plague. Without appropriate treatment, the pathogen rapidly causes septicaemia, the terminal and fatal phase of the disease. In order to identify bacterial genes which are essential during septicaemic plague in humans, we performed a transcriptome analysis on the fully virulent Y. pestis CO92 strain grown in either decomplemented human plasma or Luria-Bertani medium, incubated at either 28 or 37 degrees C and harvested at either the mid-exponential or the stationary growth phase. Y. pestis genes involved in 12 iron-acquisition systems and one iron-storage system (bfr, bfd) were specifically induced in human plasma. Of these, the ybt and tonB genes (encoding the yersiniabactin siderophore virulence factor and the siderophore transporter, respectively) were induced at 37 degrees C, i.e. under conditions mimicking the mammalian environment. Growth in human plasma also upregulated genes involved in the synthesis of five fimbrial-like structures (including the Psa virulence factor), and in purine/pyrimidine metabolism (the nrd genes). Genes known to play a role in the virulence of several bacterial pathogens (such as those encoding the Lpp lipoprotein and non-iron metal-uptake proteins) were induced in human plasma, during either the exponential or the stationary phase. Finally, 120 genes encoding proteins of unknown function were upregulated in human plasma. Eleven of these genes were specifically transcribed at 37 degrees C and may thus represent new virulence factors that are important during the septicaemic phase of human plague.

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Available from: Michael Marceau, Jun 19, 2014
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    • "The upregulation of genes required for iron acquisition and for resistance to nitrosative stress suggests that iron deprivation and NO-induced stress are more severe in the rat bubo than in the mouse lung. Chauvaux et al. (2007) have analyzed the expression profile of Y. pestis CO92 incubated in decomplemented human plasma mimicking the septicaemic plague. Dozens of genes for iron acquisition or storage systems are particularly induced in the human plasma. "
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    Frontiers in Cellular and Infection Microbiology 12/2012; 2:157. DOI:10.3389/fcimb.2012.00157 · 3.72 Impact Factor
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    • "This data infers that OmpR might play the role of a transcriptional activator for ysu gene expression , the function which is performed by AlcR for the alc genes expression. The ex vivo transcriptome analysis of Y. pestis and Y. pseudotuberculosis grown in human plasma (Chauvaux et al., 2007; Rosso et al., 2008) showed that the ysu genes were up-regulated in plasma in both species. In contrast, no difference in expression was observed in Ybt-negative Y. pestis KIM5pgm inside J774.1 macrophage-like cells (Fukuto et al., 2010). "
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    • "Y. pestis does not live freely in the soil or water, instead it typically colonizes fleas in order to be transmitted to mammals (Lorange et al., 2005), and maintains considerable genetic material dedicated to this part of its life cycle (Hinnebusch et al., 2002; Darby et al., 2005; Vadyvaloo et al., 2007; Sebbane et al., 2009; Chouikha and Hinnebusch, 2012). In addition, Y. pestis has been shown to colonize multiple mammalian organs, including the lymph nodes, spleen, lungs, and blood, and the infection of these distinct sites in the body results in the expression of different subsets of genes (Tieh and Landauer, 1948; Lathem et al., 2005; Sebbane et al., 2005; Lawson et al., 2006; Chauvaux et al., 2007). "
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