A Selective Chemical Probe for Coenzyme A-Requiring Enzymes

Department of Chemistry, University of Illinois, Urbana-Champaign, Urbana, Illinois, United States
Angewandte Chemie International Edition (Impact Factor: 11.26). 10/2007; 46(40):7621-4. DOI: 10.1002/anie.200702485
Source: PubMed


Transferasen markiert: Eine auf dem Coenzym A (CoA) basierende Affinitätssonde mit einer Sulfoxycarbamat-Funktion erkennt eine Reihe von Acetyltransferasen selektiv gegenüber anderen Enzymen und Proteinen. Es verbleibt eine Desthiobiotin-Markierung, die für Western-Blots und die massenspektrometrische Charakterisierung genutzt werden kann (siehe Bild; Nu=Nucleophil).

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