Article

[Studies on HPLC-fingerprint of Rhizoma Fagopyri Dibotoryis].

Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 07/2007; 32(12):1171-4. pp.1171-4
Source: PubMed

ABSTRACT To establish an HPLC fingerprint of Rhizoma fagopyri dibotoryis.
The HPLC-electrochemical detection assay was used to establish the fingerprint of Rhizoma fagopyri dibotoryis. The sample was performed on a column of Diamonsil C18 (4.6 mm x 250 mm, 5 microm) which was eluted with methanol- 0.1 mol x L(-1) phosphate buffer (pH 2.5), the flow rate was 1.0 mL x min(-1), the column temperature was 35 degrees C, the reference electrode was ISAAC (in-situ silver/silver chloride), the work electrode was glassy carbon, the counter electrode was Pt platmun.
The HPLC fingerprint profiles of 6 Rhizoma fagopyri dibotoryis contains 6 common chromatographic peaks, and gallic acid, protocatechuic acid, protocatechuic aldehyde and ( - ) -epicatechin were tested the samples. The contents of protocatechuic acid and protocatechuic aldehyde were from 0.004% to 0.05% and from 0.003% to 0.015%, respectively.
The method can be used to control the quality of Rhizoma fagopyri dibotoryis.

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Keywords

5 microm
 
6 common chromatographic peaks
 
6 Rhizoma fagopyri dibotoryis
 
column temperature
 
fingerprint
 
gallic acid
 
HPLC fingerprint
 
HPLC fingerprint profiles
 
HPLC-electrochemical detection assay
 
in-situ silver/silver chloride
 
protocatechuic aldehyde
 
Rhizoma fagopyri dibotoryis
 
samples