A Laccase Associated with Lignification in Loblolly Pine Xylem

Science (Impact Factor: 33.61). 05/1993; 260(5108):672-4. DOI: 10.1126/science.260.5108.672
Source: PubMed


Peroxidase has been thought to be the only enzyme that oxidizes monolignol precursors to initiate lignin formation in plants. A laccase was purified from cell walls of differentiating xylem of loblolly pine and shown to coincide in time and place with lignin formation and to oxidize monolignols to dehydrogenation products in vitro. These results suggest that laccase participates in lignin biosynthesis and therefore could be an important target for genetic engineering to modify wood properties or to improve the digestibility of forage crops.

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Available from: Ronald Sederoff, Apr 01, 2014
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    • "Chronological Development of Laccase Applications to Fiberboard Fabrication Reference Novelty Application Yoshida (1883) Discovered laccase Discovered for the first time in latex from the Japanese lacquer tree (Rhus vernicifera) Yamaguchi et al. (1992) phenol dehydrogenative polymerization Achieved bonding among woody-fibers by polymerisation Bao et al. (1993) "
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    Bioresources 07/2015; 10(3):6262-6284. DOI:10.15376/biores.10.3.Nasir · 1.43 Impact Factor
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    • "It will be interesting to see whether the assembly of lignin polymerization complex by CASPs or CASP-like proteins observed in the formation of Casparian strip is a general feature that applies to other types of lignifying cells. Similar to peroxidase, laccase (p-diphenol:dioxygen oxidoreductase , EC also oxidizes monolignols in vitro (Bao et al., 1993; Freudenberg, 1959; Richardson et al., 1997; Sterjiades et al., 1992; Takahama, 1995), and laccase-like activities have been detected in the lignifying cell walls of differentiating xylem (Bao et al., 1993; Driouich et al., 1992; Liu et al., 1994; Richardson et al., 2000). Laccases are coppercontaining extracellular glycoproteins that require O 2 as secondary substrate to oxidize various phenolic, inorganic, and/or aromatic amine substrates (Reinhammar and Malmstroem, 1981). "
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    Plant Biotechnology Journal 09/2014; 12(9). DOI:10.1111/pbi.12250 · 5.75 Impact Factor
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    • "The liquid medium was prepared according to Tien and Krik [21]. Additional Cu 2+ (final concentration of 0.05 ␮M) was added into the liquid medium to induce the laccase production [22]. Protein purification was performed according to the method reported by Endo et al. [23]. "
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