Article

CREB-mediated Bcl-2 expression in trichosanthin-induced Hela cell apoptosis.

Institute of Cell Biology, Zhejiang University, Hangzhou 310058, PR China.
Biochemical and Biophysical Research Communications (impact factor: 2.48). 12/2007; 363(1):101-5. DOI:10.1016/j.bbrc.2007.08.141 pp.101-5
Source: PubMed

ABSTRACT Bcl-2 plays a pivotal role in the control of cell death and is down-regulated in trichosanthin (TCS)-induced cell apoptosis. Because Bcl-2 expression is regulated by the transcription factor cyclic AMP response element-binding protein (CREB), we investigated the role of CREB activation in TCS-induced Hela cells apoptosis. Our results showed that TCS-caused Hela cell apoptosis was accompanied by the decrease of Bcl-2 and phosphorylated CREB protein levels. Interesting, this inhibitive effect can be abolished by the combined treatment of TCS/cAMP agonists. Furthermore, TCS-mediated Bcl-2 protein was abrogated by the suppression of CREB expression with antisense treatment, and blocking the interaction between CREB-binding protein and the Bcl-2 cyclic AMP-responsive element (CRE) by a CRE decoy oligonucleotide. Therefore, these data support the hypothesis that CREB plays a critical role in the regulation of Bcl-2 expression in TCS-induced Hela cell death.

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Keywords

Bcl-2
 
Bcl-2 cyclic AMP-responsive element
 
Bcl-2 expression
 
cell death
 
CRE decoy oligonucleotide
 
CREB activation
 
CREB expression
 
CREB-binding protein
 
data support
 
down-regulated
 
inhibitive effect
 
phosphorylated CREB protein levels
 
pivotal role
 
suppression
 
TCS)-induced cell apoptosis
 
TCS-caused Hela cell apoptosis
 
TCS-induced Hela cell death
 
TCS-induced Hela cells apoptosis
 
TCS-mediated Bcl-2 protein
 
TCS/cAMP agonists
 

Ping Wang