Article

Sulforaphane induces growth arrest and apoptosis in human ovarian cancer cells

Division of Gynecologic Oncology, Department of Obstetrics, Gynecology and Reproductive Medicine, Mount Sinai School of Medicine, 1176 Fifth Avenue, Box 1173, New York, NY 10029, USA.
Acta Obstetricia Et Gynecologica Scandinavica (Impact Factor: 1.99). 08/2007; 86(10):1263-8. DOI: 10.1080/00016340701552459
Source: PubMed

ABSTRACT Isothiocyanates (ITC) from broccoli and other cruciferous vegetables have long been shown to have chemopreventive properties, as demonstrated in cancer models in rodents. Sulforaphane (SFN) is a major ITC present in broccoli. We examined the effects of SFN on the growth of the OVCAR-3 and SKOV-3 ovarian carcinoma cell lines.
Cell cycle phase determination was performed using a Coulter flow cytometer. DNA strand breaks in apoptotic cells were measured by terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end-labelling (TUNEL).
There was a concentration dependent decrease in cell density. Approximately 50% decrease was observed after 48 h of incubation with SFN (2 μM). Analysis of cell cycle phase progression revealed a decrease in the cell populations in S and G2M phases, with an increase of G1 cell population, indicating a G1 cell cycle arrest. The degree of decrease in the replicating population was concentration and time dependent. Incubation of OVCAR-3 cells in cultures with concentrations of 2, 10 and 50 μM of SFN showed 6, 8 and 17% apoptosis, respectively. In addition, when OVCAR-3 cells were exposed to SFN for various time periods (1, 2 or 3 days), the percentage of cells undergoing apoptosis was directly proportional to the incubation period. In this regard, while 18% of the cells underwent apoptosis after 2 days, 42% of the cells showed apoptosis after 3 days of incubation.
These results clearly demonstrated an effect of SFN in inducing growth arrest and apoptosis in ovarian carcinoma cell lines.

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    • "In addition to the induction of the phase II detoxifying enzymes, SFN activates heat shock response and enhances proteasome activity through the induction of heat shock protein 27 (HSP27) expression [8], and reduces the lipopolysaccharide-induced secretion of pro-inflammatory cytokines [9] [10]. Also, SFN induces cell cycle arrest and apoptosis in many types of cancer cells, thus inhibiting tumor growth in vivo [11] [12]. Autophagy is an evolutionally conserved pathway involved in the organized elimination of proteins, organelles and invading microbes by lysosomes. "
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    FEBS Letters 06/2014; 588(17). DOI:10.1016/j.febslet.2014.06.036 · 3.34 Impact Factor
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    • "SF modulates many cancer-related events, including susceptibility to carcinogens, cell death, cell cycle, angiogenesis, invasion and metastasis (Zhang and Tang, 2007; Fimognari and Hrelia, 2007). It has been reported in many studies that SF suppresses the growth of some tumoral cells lines (Chuang et al., 2007; Matsui et al., 2007; Mi et al., 2007; Park et al., 2007; Pledgie-Tracy et al., 2007; Shan et al., 2006; Pappa et al., 2007a). These findings suggest that cell vulnerability to SFmediated apoptosis is subject to regulation by cellcycle-dependent mechanisms (Fimognari et al., 2007). "
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    • "SF modulates many cancer-related events, including susceptibility to carcinogens, cell death, cell cycle, angiogenesis, invasion and metastasis (Zhang and Tang, 2007; Fimognari and Hrelia, 2007). It has been reported in many studies that SF suppresses the growth of some tumoral cells lines (Chuang et al., 2007; Matsui et al., 2007; Mi et al., 2007; Park et al., 2007; Pledgie-Tracy et al., 2007; Shan et al., 2006; Pappa et al., 2007a). These findings suggest that cell vulnerability to SFmediated apoptosis is subject to regulation by cellcycle-dependent mechanisms (Fimognari et al., 2007). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Many cultures have used plants to treat medical affections and a percentage of modern medicines are obtained from plants. Today, several herbals have been screened for anticancer activity and many patients with cancer take plant extracts in addition to chemotherapy. This review offers an overview of the knowledge about the use of herbals and derivatives as a viable anticancer alternative therapy and their interactions (particularly, modulation of P-450 system and P-glycoprotein) with conventional anti-cancer drugs. It is suggested that health care professionals and patients should be aware of the potential for adverse interactions of this products with the anti-cancer drugs.
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