Large outbreak of viral gastroenteritis caused by contaminated drinking water in Apulia, Italy, May-October 2006.
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ABSTRACT: Determine the epidemiological profile of outbreaks of acute diarrheal disease caused by rotavirus (RV) occurring in pediatric patients, based on a critical review of the literature published between 2000 and 2010. A search was carried out for articles published from January 2000 to April 2010, collected by the Artemisa, EBSCO, Embase, Imbiomed, Lilacs, Ovid, PubMed, and Science Direct databases. In the studies that met the inclusion criteria, possible confounding factors were identified and risks of bias were attributed based on the number of items considered inadequate in each case. The epidemiological and microbiological characteristics of the outbreaks were described. The sample was comprised of only 14 (10.8%) of the 129 titles identified, which accounted for 91 092 reported cases of acute diarrhea. In 5 250 of these cases, a search for rotavirus was conducted, yielding 1 711 (32.5%) positive isolations. It was observed that the RV from Group A was the causative agent in 100% of the outbreaks, while genotype G9 was documented in 50% of the articles. Rotavirus, mainly serotype G9, was one of the principal agents responsible for outbreaks of acute diarrheal disease over the past decade. A careful outbreak study can contribute valuable information for RV disease control and prevention.Revista Panamericana de Salud Pública 02/2012; 31(2):142-7. · 0.85 Impact Factor
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ABSTRACT: Using genetically engineered lactobacilli, producing high avidity llama VHH domains (referred to as anti-rotavirus proteins; ARPs), to test the effect of multimeric antibody fragments as prophylaxis and therapy against rotavirus infection. Two ARPs, ARP1 and ARP3, shown to bind to different epitopes and act synergistically against rotavirus, were displayed on the surface of Lactobacillus paracasei as monovalent or bivalent proteins (mono- or bi-specific). Although a nonsignificant difference was observed between lactobacilli producing bispecific ARP3-ARP1 and monomeric ARPs, lactobacilli producing bispecific ARP3-ARP1 were superior at reducing the rate of diarrhea when used for prophylactic and therapeutic intervention in a mouse model of rotavirus infection in comparison to nontreated animals. Expression of bispecific antibodies in lactobacilli resulted in slight improvement of their efficacy. Furthermore, increasing the specificity would theoretically reduce the rate of appearance of viral escape mutants and would have a broader capacity to be effective against a range of viral serotypes.Future Microbiology 05/2011; 6(5):583-93. · 4.02 Impact Factor
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ABSTRACT: In the Salento peninsula (Puglia Region, South-East Italy), underground waters are a fundamental resource for the population because they constitute the principal reservoir for drinking water and irrigation. They are, however, affected by overexploitation. The risk factors in the Salento arise mainly from anthropic activities, especially tourism and agriculture (leaking wells, sewage and inadequate waste disposal procedures). The Southern Salento is recognized to be at high risk of pathologies characterised by oral-faecal transmission. From 2001 to 2009 the incidence of typhoid fever in the Salento was 12.11/100,000 inhabitants as against 2.91 in Italy. Enteritis caused by rotaviruses is an important cause of hospitalization of paediatric-aged children in the Salento, with high social costs. An effective monitoring system for the conservation and management of water bodies and the protection of public health is therefore fundamental. The present study sought to determine the microbiological and chemical-physical quality of groundwater in the Salento and to analyse the factors associated with contamination. The results indicated widespread pollution from salt and microbial contamination. Contamination from faecal microorganisms posed a significant risk of human infection in 100% of samples. Furthermore, the water was unsuitable even for irrigation in a high percentage of cases (31.8%), which is of considerable significance given that agriculture is one of the most important economic activities in the area under study. The high salt concentration was probably due to excessive extraction of water for intensive irrigation, especially in summer. Under these circumstances, some of mitigation activity is necessary. Furthermore, it would be advisable to decrease the pollution load from anthropic activities in the territory and to reduce water consumption in order to conserve groundwater resources especially.Journal of Environmental Monitoring 11/2010; 13(1):192-200. · 2.09 Impact Factor
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Eurosurveillance, Volume 12, Issue 16, 19 April 2007
Citation style for this article: Martinelli D, Prato R, Chironna M, Sallustio A, Caputi G, Conversano M, Ciofi Degli Atti ML, D’Ancona FP, Germinario CA,
Quarto M. Large outbreak of viral gastroenteritis caused by contaminated drinking water in Apulia, Italy, May - October 2006. Euro Surveill. 2007;12
(16):pii=3176. Available online: http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=3176
Large outbreak of viral gastroenteritis caused by contaminated drinking w ater in
Apulia, I taly, May - October 2006
D Martinelli1, R Prato2, M Chironna1, A Sallustio1, G Caputi2, M Conversano3, M Ciofi Degli Atti4,
FP D’Ancona4, CA Germinario (email@example.com)1, M Quarto1
1. Dipartimento di Scienze Biomediche e Oncologia Umana, Sezione di Igiene, Università di Bari;
Osservatorio Epidemiologico Regione Puglia, (Department of Biomedical Sciences, Section of
Hygiene, University of Bari; Apulia Regional Epidemiological Observatory), Bari, Italy
2. Dipartimento di Scienze Mediche e del Lavoro, Sezione di Igiene Università di Foggia
(Department of Medical Sciences, Section of Hygiene, University of Foggia), Foggia, Italy
3. Azienda Sanitaria Locale (Local Health Unit), Taranto, Italy
4. Istituto Superiore di Sanità (National Institute of Health), Rome, Italy
At the end of July 2006, an unusually high number of patients with acute diarrhoea were reported
by the accident and emergency departments in Taranto, Apulia. Subsequently, a field
investigation was conducted jointly by the Apulia Regional Epidemiological Observatory and the
Regional Reference Laboratory in Bari, and the Epidemiological Department of Taranto Local
The outbreak investigation carried out between July and October 2006, involving hospitals in the
whole province of Taranto, included the following main elements:
G Case ascertainment and descriptive epidemiology. A case was defined as a patient with
diarrhoea (at least three loose or liquid stools in a day) and one or more of the following
symptoms: fever > = 38°C, headache, vomit, abdominal pain, nausea [1,2].
Five out of six hospitals in the province of Taranto provided information on patients with
acute gastroenteritis. Data were collected retrospectively for the period between May and
July and prospectively for August and September 2006. In addition, the special medical
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facilities set up for tourists in the summer season (June-August) in the province of Taranto
were also asked to report cases.
G Microbiological investigation of stool samples of hospitalised patients.
G Microbiological investigation of environmental samples (including tap water, sea water and
G Case control study performed between 1 August and 15 September 2006 in order to
identify the possible sources of infection.
From 1 May to 30 September 2006, a total of 2,860 patients with gastroenteritis symptoms were
either admitted to hospital or seen by the hospitals’ outpatient accident and emergency units.
This significantly exceeded the number reported in the same period in 2005, when a total of 586
patients with gastroenteritis were treated by the same hospitals. The epidemic curve is shown in
Figure 1. Number of patients with gastroenteritis seeking hospital care, by week. Taranto
province, 1 May-30 September, 2005 and 2006
The first peak in incidence was observed at the end of June (26 week of the year), followed by a
second peak at the end of July (weeks 29 and 30). The number of patients with gastroenteritis
seeking hospital care decreased in the following weeks. By mid-September, the number of cases
per week was similar to that seen in the same period of 2005.
Patients mean age was 25 years; 19% of the cases were under 5 years of age, 16% were 5 to 15
years old, and 65% were above 15 years of age. Incubation time was not calculated because it
was not possible to determine the exact time of exposure.
Incidence by town of residence was highest in the city of Taranto (9.5 cases per 1,000
inhabitants) (Figure 2).
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Figure 2. Number of patients with gastroenteritis seeking hospital care, by town of residency per
1,000 inhabitants. Taranto province, 1 May-30 September 2006
Data collected by the tourist medical facilities in Taranto province showed a total of 361 cases of
acute gastroenteritis, significantly more than a year before. Hence the same trend was observed
as in the case of hospital data.
A total of 70 stool samples from patients affected by the outbreak were collected and analysed.
Results by age group are reported in Table 1.
Table 1. Stool samples collected from patients and tested by the Regional Reference Laboratory
(U.O.C. Igiene, Azienda Ospedaliera Policlinico), Bari, August – September 2006
Rotavirus ( °) Norovirus ( *)
> = 15
32 (62% )
1 (14% )
1 (8% )
34 (48% )
19 (37% )
4 (57% )
5 (41% )
28 (40% )
(°) Nested PCR in VP7 region
(*) Nested PCR in the polymerase gene
Stool samples were also examined with respect to gastrointestinal bacteria and parasites. No
samples examined were positive for the entire range of pathogens tested.
Further genotyping of the samples is currently being done.
Environmental samples, systematically collected for microbiological analyses, were tap water from
the water distribution system across the whole area affected by the outbreak, sea water and
shellfish. The water samples were collected at the local waterworks, from major water pipelines
and wells, and from tap water in pubs.
No faecal indicator bacteria and endotoxins were detected in the environmental samples of tap
water collected in Taranto city. Of 44 samples tested, four (9% ) were positive for norovirus and
11 (25% ) for rotavirus (Table 2). The tests were performed using molecular techniques.
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Table 2. Drinkable tap water samples collected and tested – Regional Reference Laboratory (U.O.
C. Igiene, Azienda Ospedaliera Policlinico), Bari, August – September 2006
4 (9% ) 11 (25% )1 (2% )
Molecular profiles of rotavirus and norovirus identified in some tap water samples were the same
as the ones found in some patients’ stool samples. Sequence analysis showed the new norovirus
strain GGII.4 2006a and rotavirus genotype G9. The laboratory investigations, however, are still
ongoing and more results are expected in the future.
Of 12 sea water samples tested, four (33% ) were positive for norovirus and one (8,3% ) for
No shell fish samples were positive for bacteria or viruses.
Case control study
A case control study was performed in order to find an association between the occurrence of
gastroenteritis and the exposure to one or more risk factors.
A case was defined as a patient with at least 3 loose or liquid stools in a day and one or more of
the following symptoms: fever > = 38°C, headache, vomit, abdominal pain, nausea.
166 cases were selected among patients treated at the accident and emergency departments of
the hospitals in Taranto province, in the period between 1 August and 15 September 2006. The
control group consisted of 146 non-hospitalised healthy individuals who during the study period
were resident in the same area as the case patients. Cases and controls were age-matched.
A standard questionnaire was used for the interview.
Risk factors which were shown to be significantly associated with the onset of acute diarrhoea/
gastroenteritis were the use of tap water (OR= 2; 95% CI: 1,23-3,36), and the use of water of
uncertain origin in the 72 hours before the onset of the symptoms (OR= 3,9; 95% CI: 1,41-
Conclusion and control measures
The epidemiological investigation and the laboratory tests showed that the possible source of
infection was the drinkable tap water contaminated with (at least) rota- and noroviruses. An extra
chlorination treatment for household water supplies was therefore performed starting from the
34th week of the year in order to stop a possible contamination of the water.
Systematic technical and microbiological investigations of the pipelines and wells of the water
distribution system did not reveal the source of contamination even though technical problems at
the local chlorination treatment facilities could not have been excluded.
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To date, the outbreak of viral gastroenteritis described in this paper is probably the largest one
associated with drinking tap water in Italy.
A. Mincuzzi, T. Battista, R. Mongelli, M.T. Balducci, C. Ladalardo, A. Fusco, S. Tafuri, A.
Panebianco, F. Fortunato, A. Arbore, L. Lamarina (Department of Biomedical Science and Human
Oncology, Hygiene Section, University of Bari - Apulia Regional Epidemiological Observatory); A.
Pesare, S. Minerba, G. Grassi, M. Rollo, D. Zuppiroli (Taranto Local Health Unit); F. Portincasa, P.
Montemurro, G. Vitucci (Apulia Water Distribution Company “Ente Acquedotto Pugliese”)
1. Chironna M, Prato R. Lopalco PL, Germinario C, Sallustio A, Barbuti S, Quarto M. Norovirus GI e GII in corso
di epidemia di gastroenterite acuta associata al consumo di molluschi bivalvi in Puglia. Rapporti ISTISAN
2003; 3/C5: 39.
2. Prato R, Lopalco PL, Chironna M, Barbuti G, Germinario C, Quarto M. Norovirus gastroenteritis general
outbreak associated with raw shellfish consumption in South Italy. BMC Infectious Diseases, 2004; 4: 37.
3. Liang JL, Dziuban EJ, Craun GF, Hill V, Moore MR, Gelting RJ, Calderon RL, Beach MJ, Roy SL. Surveillance
for waterborne disease and outbreaks associated with drinking water and water not intended for drinking,
United States, 2003-2004. In: Surveillance Summaries, MMWR, 2006; 55: 31-58.
4. Boccia D, Tozzi AE, Cotter B, Rizzo C, Russo T, Buttinelli G, Caprioli A, Marziano ML, Ruggeri FM.
Waterborne Outbreak of Norwalk-Like Virus Gastroenteritis at a Tourist Resort, Italy. Emerging Infection
Diseases, 2002; 8(6):563-568.
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