Inflammatory responses induced by interleukin-17 family members in human colonic subepithelial myofibroblasts.
ABSTRACT We investigated the potential role of interleukin (IL)-17 family members (IL-17A to IL-17F) in the induction of inflammatory responses in human colonic subepithelial myofibroblasts (SEMFs).
The expression of the inflammatory cytokines IL-6, IL-8, leukemia inhibitory factor (LIF), and matrix metalloproteinases (MMP)-1 and MMP-3 were evaluated by enzyme-linked immunosorbent assay and Northern blotting. Activation of mitogen-activated protein kinase (MAPK) was assessed by immunoblotting.
IL-17A and IL-17F significantly enhanced IL-6, IL-8, LIF, MMP-1, and MMP-3 secretion. The effects of IL-17A were relatively stronger than those induced by IL-17F. The effects of IL-17B, IL-17C, IL-17D, and IL-17E were modest as compared with those induced by IL-17A and IL-17F. Both IL-17A and IL-17F augmented IL-1beta-induced secretion of IL-6, IL-8, LIF, MMP-1, and MMP-3. A similar augmentation was also observed in tumor necrosis factor (TNF)-alpha-induced cytokine and MMP secretion. IL-17A and IL-17F rapidly induced phosphorylation of extracellular signal-regulated kinases (ERK) 1/2, p38 MAPKs, and c-Jun-NH(2)-terminal kinase (JNK) as early as 15 min after stimulation. Inhibitors for ERK (PD98059 and U0216) and p38 MAPK (SB203580) significantly reduced the IL-17F-induced IL-6, IL-8, LIF, MMP-1, and MMP-3 secretion.
Among IL-17 family members, IL-17A and IL-17F strongly stimulate human colonic SEMFs, inducing inflammatory responses.
Conference Paper: Implementation of a bi-directional DC-DC converter in FCEV[Show abstract] [Hide abstract]
ABSTRACT: This article presents the FCEV power supply system configuration scheme, and proposes a topology of high power bi-directional DC-DC converter suited to FCEV. The operating principle of the converter is analyzed thoroughly. In order to guarantee the power flowing smoothly in both directions, a mode-switching unit is designed and a novel mode switching method is proposed. A 5 kW bi-directional DC-DC converter is constructed, which is controlled by the DSP-TI320F243. In addition, the design of circuit parameters and power devices are discussed. The experimental results show that the converter have excellent electrical characteristics, and it can be applied in the EV as well as the distributed power station and energy storage system.Electrical Machines and Systems, 2003. ICEMS 2003. Sixth International Conference on; 12/2003
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ABSTRACT: A hot wall-based low temperature annealing system using resistively heated, stacked hot plates was designed and tested for low temperature (100~500degC) annealing applications for 200 mm and 300 mm wafers. The system is designed to process five wafers simultaneously for productivity enhancement purposes. Thermal properties of the system and wafer temperature profiles during low temperature annealing in stacked hot plates were characterized as a function of hot plate temperature. The stacked hot plate configuration with proper gap between wafer and surrounding hot plates makes convection heat transfer predominant and provides uniform and repeatable process results in the low temperature region. Process uniformity and repeatability of NiSi formation, Cu annealing, Al sintering, spin-on-dielectrics (SOD) anneal were confirmed in the temperature range of 100~500degCAdvanced Thermal Processing of Semiconductors, 2004. RTP 2004. 12th IEEE International Conference on; 02/2004
Conference Paper: Dasymetric mapping with object-oriented classification and GISGeoscience and Remote Sensing Symposium, 2005. IGARSS '05. Proceedings. 2005 IEEE International; 08/2005