Article

Immunolocalization of a lipase-like protein in the reproductive apparatus of female Phlebotomus papatasi, at various stages of the gonotrophic cycle.

Dipartimento di Scienze Ambientali, Largo dell'Università, Università della Tuscia, 01100 Viterbo, Italy.
Annals of Tropical Medicine and Parasitology (impact factor: 1.43). 11/2007; 101(7):611-9. DOI:10.1179/136485907X193879 pp.611-9
Source: PubMed

ABSTRACT In female phlebotomine sandflies, little is known about the reproductive accessory glands that presumably contribute to egg production and/or oviposition. The main protein secreted in the accessory glands of female Phlebotomus papatasi was recently characterised as a lipase-like protein, the first to be found in the female accessory glands of any insect. This protein, named PhpaLIP (for Phlebotomus papatasi lipase), has now been detected and localized in the reproductive tissues of female P. papatasi, at different stages of the gonotrophic cycle, using a polyclonal anti-PhpaLIP serum and both confocal scanning laser and immuno-electron microscopy. PhpaLIP appears to be always present in the accessory glands (with a secretory peak shortly before oviposition) but was also detected in the follicle cells of the ovarioles, within the developing vitelline envelope, and in the oviducts. The results are discussed in relation to the functions that PhpaLIP could have during the gonotrophic cycle, in the various reproductive structures of female P. papatasi.

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    Article: First transcriptome of the testis-vas deferens-male accessory gland and proteome of the spermatophore from Dermacentor variabilis (Acari: Ixodidae).
    Daniel E Sonenshine, Brooke W Bissinger, Noble Egekwu, Kevin V Donohue, Sayed M Khalil, R Michael Roe
    [show abstract] [hide abstract]
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    PLoS ONE 01/2011; 6(9):e24711. · 4.09 Impact Factor
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Keywords

accessory glands
 
confocal scanning laser
 
developing vitelline envelope
 
different stages
 
egg production
 
female accessory glands
 
female P. papatasi
 
female phlebotomine sandflies
 
female Phlebotomus papatasi
 
follicle cells
 
gonotrophic cycle
 
immuno-electron microscopy
 
lipase-like protein
 
main protein secreted
 
Phlebotomus papatasi lipase
 
polyclonal anti-PhpaLIP serum
 
reproductive accessory glands
 
reproductive tissues
 
secretory peak
 
various reproductive structures