Engineered alkane-hydroxylating cytochrome P450(BM3) exhibiting nativelike catalytic properties.

Department of Chemistry and Chemical Engineering, California Institute of Technology, 1200 California Blvd. MC 210-41, Pasadena, CA 91125, USA.
Angewandte Chemie International Edition (Impact Factor: 11.34). 02/2007; 46(44):8414-8. DOI: 10.1002/anie.200702616
Source: PubMed
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    ABSTRACT: Although catalytic reductions, cross‐couplings, metathesis, and oxidation of CC double bonds are well established, the corresponding catalytic hydroxylations of CH bonds in alkanes, arenes, or benzylic (allylic) positions, particularly with O2, the cheapest, “greenest”, and most abundant oxidant, are severely lacking. Certainly, some promising examples in homogenous and heterogenous catalysis exist, as well as enzymes that can perform catalytic aerobic oxidations on various substrates, but these have never achieved an industrial‐scale, owing to a low space‐time‐yield and poor stability. This review illustrates recent advances in aerobic oxidation catalysis by discussing selected examples, and aims to stimulate further exciting work in this area. Theoretical work on catalyst precursors, resting states, and elementary steps, as well as model reactions complemented by spectroscopic studies provide detailed insight into the molecular mechanisms of oxidation catalyses and pave the way for preparative applications. However, O2 also poses a safety hazard, especially when used for large scale reactions, therefore sophisticated methodologies have been developed to minimize these risks and to allow convenient transfer onto industrial scale.
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    ABSTRACT: Terminal oxidant‐supported P450 reactions alleviate the need for substrate binding to initiate catalysis by chemically generating “compound I.” This allows investigation of the innate substrate range of the enzyme active site. Using iodosylbenzene as the oxidant, CYP153A6, a medium‐chain terminal alkane hydroxylase, exhibits methanol formation in the presence of methane demonstrating that P450‐mediated methane hydroxylation is possible.
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