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Engineered Alkane-Hydroxylating Cytochrome P450BM3 Exhibiting Nativelike Catalytic Properties

Department of Chemistry and Chemical Engineering, California Institute of Technology, 1200 California Blvd. MC 210-41, Pasadena, CA 91125, USA.
Angewandte Chemie International Edition (Impact Factor: 11.26). 02/2007; 46(44):8414-8. DOI: 10.1002/anie.200702616
Source: PubMed

ABSTRACT (Figure Presented) Divide, evolve, and conquer: A domain-based strategy (see scheme) was used to engineer high catalytic and coupling efficiency for propane hydroxylation in a multidomain cytochrome P450 enzyme. The engineered enzymes exhibit high total activities in whole-cell bioconversions of propane to propanol under mild conditions, using air as oxidant.

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Available from: Rudi Fasan, Jul 28, 2015
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