Article

Protective effect of phlorotannin components phloroglucinol and eckol on radiation-induced intestinal injury in mice.

College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, South Korea.
Phytotherapy Research (impact factor: 2.09). 03/2008; 22(2):238-42. DOI:10.1002/ptr.2298 pp.238-42
Source: PubMed

ABSTRACT Components of phlorotannin, which were extracted from Ecklonia species, have been reported to have in vitro radioprotective and antioxidative effects. The radioprotective effects of two of the components of phlorotannin, phloroglucinol and eckol, in intestinal stem cells were examined by evaluating their effects on jejunal crypt survival and apoptosis in gamma-irradiated mice. Pretreating the mice (i.p. 20 mg/kg of body weight at 12 and 36 h before irradiation) prior to irradiation with either phloroglucinol or eckol significantly improved the survival of the jejunal crypt (p < 0.001 and p < 0.01 vs irradiation controls at 3.5 days after 8 Gy irradiation, respectively). The administration of phloroglucinol and eckol prior to irradiation protected the intestinal crypts from radiation-induced apoptosis (p < 0.05 vs irradiation controls at 12 h after 1 Gy irradiation). Although the mechanism for this inhibitory effect remains unknown, these results showed that phloroglucinol and eckol might be useful radioprotectors that can defend intestinal stem cells against the oxidative damage caused by gamma-irradiation.

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Keywords

1 Gy irradiation
 
8 Gy irradiation
 
antioxidative effects
 
apoptosis
 
Components
 
Ecklonia species
 
eckol
 
gamma-irradiated mice
 
gamma-irradiation
 
inhibitory effect
 
intestinal crypts
 
irradiation
 
irradiation controls
 
jejunal crypt
 
jejunal crypt survival
 
oxidative damage
 
phlorotannin
 
radiation-induced apoptosis
 
radioprotective effects
 
vitro radioprotective