Measurement of dynamic mobilization of trace metals in sediments using DGT and comparison with bioaccumulation in Chironomus riparius: First results of an experimental study

Cemagref, Research unit Water Quality, 3 bis quai Chauveau, CP220, F-69336 Lyon cedex 09, France.
Chemosphere (Impact Factor: 3.34). 02/2008; 70(5):925-32. DOI: 10.1016/j.chemosphere.2007.06.061
Source: PubMed


Sediments in aquatic ecosystems are often contaminated as a result of anthropogenic activities. Sediments and benthic organisms have been used to monitor trace metals contamination. However, due to the high variability of contaminant bioavailability, the attempt to link metal concentration in sediments and contamination of the organisms or ecotoxicological effect often lead to disappointing results. The technique of diffusive gradients in thin films (DGT) has been proposed as a relevant tool to study metal bioavailability, for example for accumulation in plants. In the present study, laboratory microcosm experiments were conducted with six contaminated sediments to compare metal accumulation in DGT and bioaccumulation in a chironomid (Chironomus riparius) for Cu, Cd and Pb . Metal accumulation in DGT was measured over time then modelled to determine two parameters of the dynamic response of the metals to DGT deployment: the size of the particulate labile pool and the kinetic of the solid-dissolved phase exchange. The mobility of metals was found metal and sediment dependent. A significant relationship between metal accumulated in DGT and bioaccumulated in chironomids was found for Cu and Pb. However, total metals in sediments were the best predictors of bioaccumulation. Nevertheless, the knowledge of the metals dynamic enhanced our ability to explain the different biological uptake observed in sediments of similar total metal concentrations.

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    • "To date, few studies have been carried out to assess the bioavailability of metals measured by DGT to the tested organisms in field sediment toxic studies. For example, Roulier et al. [23] [24] found that total metal and DGT measurement were similar, but that the DGT technique was inferior in a laboratory simulated sediment toxicity study. Simpson et al. [25] indicated that the DGT-induced flux could predict bioaccumulation and toxicity to bivalves in sediments with varying properties well. "
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    ABSTRACT: In this study, we used the diffusive gradients in thin films (DGT) and conventional methods (including SEM-AVS models, BCR sequential extraction and total metal concentrations) to assess sediment Cr, Ni, Cu, Zn, Cd and Pb bioavailability to field inhabitant freshwater snails (Bellamya aeruginosa) from Chinese eutrophic lakes. The performance of these methods and the relationship between DGT measurements and conventional methods were evaluated. The results showed that DGT-measured metal concentrations have weak correlations with results from tests using SEM-AVS models as well as sequentially extracted European Community Bureau of Reference (BCR) metal fractions. Among the methods used, Cr, Ni, Cu, and Pb measured by DGT were significantly correlated with metal concentrations in the tissue of snails, while SEM-AVS could predict Cr, Ni and Pb bioavailability well, but not SEM-AVS/fOC. Finally, BCR sequential extraction and total metal concentrations only correlated well with Pb bioavailability to snails. Overall, the results of this study indicated that DGT performed best in predicting metal accumulation in snails and which could be used to predict sediment metal bioavailability to field inhabitant snails from freshwater lake sediments due to their simple manipulation and validity.
    Journal of hazardous materials 11/2013; 264C:184-194. DOI:10.1016/j.jhazmat.2013.11.030 · 4.53 Impact Factor
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    • "These predictions do not account for the buffer capacity of the soil, i.e., its ability to replenish the supply to the porewater (Nowack et al., 2004). This method was applied for correlating the metal uptake by plants (Black et al., 2011; Perez and Anderson, 2009; Roulier et al., 2008). It was also used in in vitro unified barge method (UBM) solution containing Pb, Zn and Cu to predict the heavy metals accumulated through the intestine in human body (Pelfrene et al., 2011). "
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    ABSTRACT: The applicability of diffusive gradients in thin-films (DGT) as a biomimic surrogate was investigated to determine the bioavailable heavy metal concentrations to earthworm (Eisenia foetida). The relationships between the amount of DGT and earthworm uptake; DGT uptake and the bioavailable concentrations of heavy metals in soils were evaluated. The one-compartment model for the dynamic uptake of heavy metals in the soil fitted well to both the earthworm (R(2)=0.641-0.990) and DGT (R(2)=0.473-0.998) uptake data. DGT uptake was linearly correlated with the total heavy metal concentrations in the soil (aqua regia), the bioavailable heavy metal concentrations estimated by fractions I+II of the standard measurements and testing (SM&T) and physiologically based extraction test (PBET, stomach+intestine). The coefficients of determination (R(2)) of DGT uptake vs. aqua regia were 0.433, 0.929 and 0.723; vs. SM&T fractions (I+II) were 0.901, 0.882 and 0.713 and vs. PBET (stomach+intestine) were 0.913, 0.850 and 0.649 for Pb, Zn and Cu, respectively. These results imply that DGT can be used as a biomimic surrogate for the earthworm uptake of heavy metals in contaminated soils as well as predict bioavailable concentrations of heavy metals estimated by SM&T (I+II) and PBET as a human oral bioavailable concentrations of heavy metals.
    Science of The Total Environment 11/2011; 416:127-36. DOI:10.1016/j.scitotenv.2011.11.007 · 4.10 Impact Factor
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    ABSTRACT: this article). Even before editing the first newsletter, Fittkau was one of the initiators of another way to exchange information, that chironomid workers from all around the world have enjoyed ever since as a major attraction and institution in our community: in the fall of 1963 Fittkau sent out the invitations for the first International Symposium on Chironomidae, which then took place at Pln in July of 1964. By now we are looking forward to the fifteenth such meeting, to be hosted by Len Ferrington and colleagues at the University of Minnesota in the summer of 2003. Knowing Professor Fittkau today, one can assume that meeting others and bridging distances between people or territories have always been enjoyable and rewarding to him in themselves, not just necessary means to a professional end. Nevertheless, it is fair to assume that this inclination was reinforced by positive experience made in his younger years. As a beginning student of biology, he was able to take part in the creation of the Limnologische Fluss-Station Freudenthal -- a precursor of the current Max-Planck-Institute at Schlitz -- which could only be achieved through the collective effort of its founders overcoming the most adverse post-war circumstances. The `down' side of joining this group for Fittkau -- lucky for us -- was that he was directed away from the molluscs he had wanted to study, and instead had to work himself into the Chironomidae. (However, Fittkau never has been `converted' completely, as can be seen from his wonderful collection of shells, and from the occasional papers on molluscs recurring here and there among his many publications.) The fauna of the Fulda river, which the Freudenthal group was mainly studying, was targeted as the topic of Fittkau's doctoral dissertation, and the...
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