Article

Pseudovitamin B(12) is the corrinoid produced by Lactobacillus reuteri CRL1098 under anaerobic conditions.

Kluyver Centre for Genomics of Industrial Fermentation, TI Food and Nutrition, and NIZO Food Research, Kernhemseweg 2, P.O. Box 20, 6710 BA Ede, The Netherlands.
FEBS Letters (impact factor: 3.54). 11/2007; 581(25):4865-70. DOI:10.1016/j.febslet.2007.09.012 pp.4865-70
Source: PubMed

ABSTRACT We have reported previously on the ability of Lactobacillus reuteri to produce a compound with vitamin B(12) activity. Here we report on the chemical characterisation of this corrinoid-like molecule. High performance liquid chromatography coupled to an ultraviolet diode array detector, mass spectrometry and nuclear magnetic resonance spectroscopy has enabled us to identify the compound as Coalpha-[alpha-(7-adenyl)]-Cobeta-cyanocobamide or pseudovitamin B(12). This molecule differs from cobalamin in the alpha-ligand, where it has adenine instead of 5,6-dimethylbenzimidazole bound in a alpha-glycosidic linkage to C-1 of ribose. L. reuteri is the first lactic acid bacterium in which the production of a cobalamin-like molecule has been identified and the first microorganism reported to produce exclusively pseudo-B(12).

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Keywords

5,6-dimethylbenzimidazole
 
alpha-glycosidic linkage
 
chemical characterisation
 
Coalpha-[alpha-(7-adenyl)]-Cobeta-cyanocobamide
 
cobalamin-like molecule
 
corrinoid-like molecule
 
first lactic acid bacterium
 
first microorganism
 
L. reuteri
 
Lactobacillus reuteri
 
performance liquid chromatography
 
ultraviolet diode array detector