Functionalized Carbon Nanotubes for Detecting Viral Proteins

William Penn University, Filadelfia, Pennsylvania, United States
Nano Letters (Impact Factor: 13.59). 11/2007; 7(10):3086-91. DOI: 10.1021/nl071572l
Source: PubMed


We investigated the biocompatibility, specificity, and activity of a ligand-receptor-protein system covalently bound to oxidized single-walled carbon nanotubes (SWNTs) as a model proof-of-concept for employing such SWNTs as biosensors. SWNTs were functionalized under ambient conditions with either the Knob protein domain from adenovirus serotype 12 (Ad 12 Knob) or its human cellular receptor, the CAR protein, via diimide-activated amidation. We confirmed the biological activity of Knob protein immobilized on the nanotube surfaces by using its labeled conjugate antibody and evaluated the activity and specificity of bound CAR on SWNTs, first, in the presence of fluorescently labeled Knob, which interacts specifically with CAR, and second, with a negative control protein, YieF, which is not recognized by biologically active CAR proteins. In addition, current-gate voltage (I-V(g)) measurements on a dozen nanotube devices explored the effect of protein binding on the intrinsic electronic properties of the SWNTs, and also demonstrated the devices' high sensitivity in detecting protein activity. All data showed that both Knob and CAR immobilized on SWNT surfaces fully retained their biological activities, suggesting that SWNT-CAR complexes can serve as biosensors for detecting environmental adenoviruses.

Download full-text


Available from: a t charlie Johnson, Jul 22, 2014
    • "Email: 2005; Rastogi et al., 2012; Shim, Shi Kam, Chen, Li, & Dai, 2002; Shin et al., 2008; Singh et al., 2009; Singh et al., 2005; Strano et al., 2003; Sun, Fu, Lin, & Huang, 2002; Veetil & Ye, 2007; Wang, Iqbal, & Mitra, 2005; Wang et al., 2012; Williams, Veenhuizen, de la Torre, Eritja, & Dekker, 2002; Xu, Pehrsson, Chen, Zhang, & Zhao, 2007; Yang et al., 2008; Yarotski et al., 2009; Zhang et al., 2007; Zheng, Jagota, Semke, et al., 2003; Zheng, Jagota, Strano, et al., 2003). Both organic and inorganic molecules have been employed to functionalize CNTs through covalent (Balasubramanian & Burghard, 2005; Banerjee et al., 2005; Dwyer et al., 2002; Hu et al., 2004; Jacobs et al., 2010) and non-covalent interactions (Chen et al., 2003; Zheng, Jagota, Semke, et al., 2003). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Abstract Functionalized carbon nanotubes (CNTs) constitute a new class of nano-structured materials that have vast applications in CNT purification and separation, biosensing, drug delivery, etc. Hybrids formed from the functionalization of CNT with biological molecules have shown interesting properties and have attracted great attention in recent years. Of particular interest is the hybridization of single or double stranded nucleic acid (NA) with CNT. Nucleobases, as the building blocks of NA, interact with CNT and contribute strongly to the stability of the NA-CNT hybrids and their properties. In this work, we present a thorough review of previous studies on the binding of nucleobases with graphene and CNT, with a focus on the simulation works that attempted to evaluate the structure and strength of binding. Discrepancies among these works are identified, and factors that might contribute to such discrepancies are discussed.
    Journal of biomolecular Structure & Dynamics 08/2014; 33(7):1-85. DOI:10.1080/07391102.2014.954315 · 2.92 Impact Factor
  • Source
    • "The molecular mechanism by which atomic interactions at the surface of the carbon nanotube influence the mobility of carriers within the carbon lattice is a subject of great current interest [78] directly relevant to understanding the mode of odorant sensing by DNA-coated nanotubes [79]. All-atom molecular dynamics studies of DNA-carbon nanotube interactions show that a pi-pi stacking interaction between the DNA bases and the nanotube sidewall guides the DNA-nanotube interaction [34], which is sufficiently strong [80] to allow sensing functions to occur in aqueous solution [81]. Specific dominant conformations have been identified through the use of replica exchange molecular dynamics (REMD) [82] . "
    [Show abstract] [Hide abstract]
    ABSTRACT: The analysis of breath and body odors can provide valuable information relevant to disease detection, diagnosis and treatment. A variety of technical developments are being pursued to develop electronic devices intended to analyze volatile components of breath and body odors with the sensitivity, selectivity, and learning ability of high-end mammalian olfactory systems. Here, we describe a new sensor technology that has the potential to supply a large set of diverse and sensitive odorant sensors with electronic readout to provide information-rich odorant-elicited signals for analysis by pattern recognition algorithms. In addition, we demonstrate that these sensors can provide discrimination of odorant homologues consisting of aldehydes and organic acids commonly found in human breath and other body emanations over a range of concentrations.
    IEEE Sensors Journal 02/2010; 10(1-10):159 - 166. DOI:10.1109/JSEN.2009.2035670 · 1.76 Impact Factor
  • Source
    • "Since the discovery of carbon nanotubes (CNTs) in 1991 [1], CNTs have raised considerable attention due to their fascinating structures and properties (electronic, optical, thermal, mechanical etc.) [2] [3]. Recently, its potential application in biotechnology has attracted much interest, as CNTs have been reported to exhibit great advantages in biosensors [4] [5], biomedical devices [6] and drug delivery systems (DDS) [7] [8] etc. Pristine CNTs are highly hydrophobic, so the main obstacle in the utilization of CNTs in biological and medicinal chemistry is their poor solubility in aqueous-based biological milieu. Biomolecule functionalization is one option to overcome such defects. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Non-covalent adsorption of proteins onto carbon nanotubes is important to understand the environmental and biological activity of carbon nanotubes as well as their potential applications in nanostructure fabrication. In this study, the adsorption dynamics and features of a model protein (the A sub-domain of human serum albumin) onto the surfaces of carbon nanotubes with different diameters were investigated out by molecular dynamics simulation. The adsorption behaviors were observed by both trajectory and quantitative analyses. During the adsorption process, the secondary structures of alpha-helices in the model protein were slightly affected. However, the random coils connecting these alpha-helices were strongly affected and this made the tertiary structure of protein change. The conformation and orientation selection of the protein were induced by the properties and the texture of surfaces indicated by the interaction curve. In addition, the stepwise adsorption dynamics of these processes are found. The mechanism of induced stepwise conformational change of protein on carbon nanotube surfaces would be helpful to better understand the protein-surface interaction at the molecular level.
    Biomaterials 08/2008; 29(28):3847-55. DOI:10.1016/j.biomaterials.2008.06.013 · 8.56 Impact Factor
Show more

Similar Publications