Effect of donor age on success of spermatogenesis in feline testis xenografts.

Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Reproduction Fertility and Development (Impact Factor: 2.58). 02/2007; 19(7):869-76. DOI: 10.1071/RD07056
Source: PubMed

ABSTRACT Ectopic xenografting of 'donor' feline testicular tissue into a 'recipient' immunodeficient mouse is a promising tool to preserve the male genome from genetically valuable felids. To define parameters under which the technique can succeed, we compared the effect of donor age on xenograft spermatogenesis among four age groups of domestic cats (Felis catus; age range 8 weeks to 15 months). In all cases, fresh tissue was grafted into castrated mice and collected 10, 30 and 50 weeks later. The percentage of xenografts recovered decreased as donor age increased. Mature testicular spermatozoa were observed in xenografts from the 8 and 9-16 week age groups; only a single 7-month-old donor produced elongating spermatids and xenografts from donors >/= 8 months of age degenerated. Seminal vesicle weight, an indicator of bioactive testosterone, was not significantly different between donors aged 8 weeks to 7 months and controls, suggesting that xenograft Leydig cells were ultimately functional even in the 5-7 month age group. Regardless of donor age, production of mature spermatozoa from xenografts was markedly delayed compared with controls. Comparison of xenografts that produced sperm with normal controls revealed a decrease in tubule cross-sections having post-meiotic germ cells. Together, these results indicate that the maximum practical donor age was just before the onset of puberty and that even successful xenografts had abnormalities in spermatogenesis.

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    ABSTRACT: The allografting or xenografting of testis tissue pieces into immunodeficient host mice is a new approach for germ cell conserving in livestock production as well as in human medicine. This technique also presents a new approach as a functional assay to study the spermatogenesis process and physiology in domesticated animals and humans; however, some problems observed in xenografted testis tissues remain to be unknown. Xenografts develop a dilated lumen in their seminiferous tubules during grafting period, particularly in xenografting. In addition, xenografts especially have a marked degeneration in advanced meiotic germ cells at the beginning of the grafting and spermatogenesis is reinitiated from spermatogonia stem cells or differentiating spermatogonia. In some species such as human and marmoset, this technique does not result in a complete spermatogenesis, indicating that outcomes of the allografting or xenografting of the testis tissue change according to the species. Despite these main challenges of xenografting of testis tissue, this technique implies a new approach to investigate spermatogenesis process and to solve the infertility problems both in livestock production and human medicine in the future.
    Kafkas Üniversitesi Veteriner Fakültesi Dergisi 01/2010; 16(6):1073-1077. · 0.29 Impact Factor
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    ABSTRACT: Spermatogenesis is a dynamic and complex process that involves endocrine and testicular factors. During xenotransplantation of testicular tissue fragments into immunodecifient mouse mice a functional communication between host brain and donor testis is established. This interaction allows for the progression of a normal spermatogenesis and recovery of fertilization-competent spermatozoa from a broad range of mammalian species. In the last years, significant progression have has been achieved in testis tissue xenografting that improves our knowledge about factors determining the success of grafting. The goal of this review is to provide up to date information about the role of factors such as donor age, donor species, testis tissue preservation or type of recipient mouse on the efficiency of this technique. Applications will be are described and compared with other techniques with similar purposes. Recent works demonstrates that testicular tissue xenografting is being used as a model to study gonadotoxicity of drugs and to obtain sperm from valuable young males.
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May 23, 2014