Human mesenchymal stem cells isolated from the umbilical cord

School of Medical Technology, Centre for Clinical Laboratory Medicine of Affiliated Hospital, Jiangsu University, Zhenjiang Key Institute of Clinical Laboratory Medicine, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, PR China.
Cell Biology International (Impact Factor: 1.93). 02/2008; 32(1):8-15. DOI: 10.1016/j.cellbi.2007.08.002
Source: PubMed


Mesenchymal stem cells (MSCs) are known as a population of multi-potential cells able to proliferate and differentiate into multiple mesodermal tissues including bone, cartilage, muscle, ligament, tendon, fat and stroma. In this study human MSCs were successfully isolated from the umbilical cords. The research characteristics of these cells, e.g., morphologic appearance, surface antigens, growth curve, cytogenetic features, cell cycle, differentiation potential and gene expression were investigated. After 2weeks of incubation, fibroblast-like cells appeared to be dominant. During the second passage the cells presented a homogeneous population of spindle fibroblast-like cells. After more than 4months (approximately 26 passages), the cells continued to retain their characteristics. Flow cytometry analysis revealed that CD29, CD44, CD95, CD105 and HLA-I were expressed on the cell surface, but there was no expression of hematopoietic lineage markers, such as CD34, CD38, CD71 and HLA-DR. Chromosomal analysis showed the cells kept a normal karyotype. The cell cycle at the third passage showed the percentage of G(0)/G(1), G(2)/M and S phase were 88.86%, 5.69% and 5.45%, respectively. The assays in vitro demonstrated the cells exhibited multi-potential differentiation into osteogenic and adipogenic cells. Both BMI-1 and nucleostemin genes, expressed in adult MSCs from bone marrow, were also expressed in umbilical cord MSCs. Here we show that umbilical cords may be a novel alternative source of human MSCs for experimental and clinical applications.

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Available from: Fei Mao, Mar 24, 2014
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    • "The fresh umbilical cord tissues were collected from healthy puerperal mothers after written informed consent was obtained. MSCs were isolated and characterized as previously described [18]. All experiment protocols were approved by the Ethics Committee of Jiangsu University. "
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    • "Mesenchymal stem cells (MSCs) are non-hematopoietic multipotent cells that may be isolated and expanded from a number of different sources, including bone marrow and adipose tissue (2,3). They are defined by a series of specific cell surface antigens (4) and an inborn ability to differentiate along multiple lineages, including into osteoblasts, chondrocytes and adipocytes (5–8). Although MSCs have a primary role in tissue regeneration (9,10), they also possess the ability to migrate to the site of numerous tumor types in vivo(11,12). "
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    • "Fresh umbilical cords were obtained from consenting mothers, and processed within 6 h. HucMSCs were isolated as previously described [24]. All cords were cultured in low glucose Dulbecco’s modified Eagle’s medium (LG-DMEM) containing 10% fetal bovine serum (FBS; Shanghai ExCell Biology, China) and 1% penicillin and streptomycin at 37°C with 5% CO2. "
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