Anaerobic Elemental Sulfur Reduction by Fungus Fusarium oxysporum
Reduction of inorganic sulfur compounds by the fungus Fusarium oxysporum was examined. When transferred from a normoxic to an anoxic environment, F. oxysporum reduced elemental sulfur to hydrogen sulfide (H2S). This reaction accompanied fungal growth and oxidation of the carbon source (ethanol) to acetate. Over 2-fold more of H2S than of acetate was produced, which is the theoretical correlation for the oxidation of ethanol to acetate. NADH-dependent sulfur reductase (SR) activity was detected in cell-free extracts of the H2S-producing fungus, and was found to be up-regulated under the anaerobic conditions. On the other hands both O2 consumption by the cells and cytochrome c oxidase activity by the crude mitochondrial fractions decreased. These results indicate that H2S production involving SR was due to a novel dissimilation mechanism of F. oxysporum, and that the fungus adapts to anaerobic conditions by replacing the energy-producing mechanism of O2 respiration with sulfur reduction.
Available from: ncbi.nlm.nih.gov
- "Analytical measurements of metabolism have revealed anoxic NH 3 fermentation is widely conserved amongst other soil-dwelling fungi (Zhou et al. 2002), but it is not clear whether most of these species can use NH 3 -fermentation as a viable strategy for long-term increases in biomass or if it more usefully provides a neat short-term adaptation to anoxia. Fusarium oxysporum is also able to grow using sulphur as an exogenous electron acceptor, too, in a pathway that is likely to share the same enzymes for ATP production as NH 3 fermentation (Abe et al. 2007; Zhou et al. 2002). "
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ABSTRACT: Protists account for the bulk of eukaryotic diversity. Through studies of gene and especially genome sequences the molecular basis for this diversity can be determined. Evident from genome sequencing are examples of versatile metabolism that go far beyond the canonical pathways described for eukaryotes in textbooks. In the last 2-3 years, genome sequencing and transcript profiling has unveiled several examples of heterotrophic and phototrophic protists that are unexpectedly well-equipped for ATP production using a facultative anaerobic metabolism, including some protists that can (Chlamydomonas reinhardtii) or are predicted (Naegleria gruberi, Acanthamoeba castellanii, Amoebidium parasiticum) to produce H(2) in their metabolism. It is possible that some enzymes of anaerobic metabolism were acquired and distributed among eukaryotes by lateral transfer, but it is also likely that the common ancestor of eukaryotes already had far more metabolic versatility than was widely thought a few years ago. The discussion of core energy metabolism in unicellular eukaryotes is the subject of this review. Since genomic sequencing has so far only touched the surface of protist diversity, it is anticipated that sequences of additional protists may reveal an even wider range of metabolic capabilities, while simultaneously enriching our understanding of the early evolution of eukaryotes.
Protist 10/2010; 161(5):642-71. DOI:10.1016/j.protis.2010.09.001 · 3.05 Impact Factor
Available from: William Martin
- "This puzzle of the production of acetate does not, however, have too many biochemical parts, as only a handful of enzymes is involved . There are of course cases where eukaryotic acetate production might harbour additional surprises, for example in the ascomycete Fusarium oxysporum, that will grow under anaerobic conditions on a variety of reduced carbon sources using elemental sulphur as the terminal electron acceptor, generating H 2 S as the reduced end product in a 2:1 molar ratio relative to acetate (Abe et al., 2007). "
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ABSTRACT: Formation and excretion of acetate as a metabolic end product of energy metabolism occurs in many protist and helminth parasites, such as the parasitic helminths Fasciola hepatica, Haemonchus contortus and Ascaris suum, and the protist parasites, Giardia lamblia, Entamoeba histolytica, Trichomonas vaginalis as well as Trypanosoma and Leishmania spp. In all of these parasites acetate is a main end product of their energy metabolism, whereas acetate formation does not occur in their mammalian hosts. Acetate production might therefore harbour novel targets for the development of new anti-parasitic drugs. In parasites, acetate is produced from acetyl-CoA by two different reactions, both involving substrate level phosphorylation, that are catalysed by either a cytosolic acetyl-CoA synthetase (ACS) or an organellar acetate:succinate CoA-transferase (ASCT). The ACS reaction is directly coupled to ATP synthesis, whereas the ASCT reaction yields succinyl-CoA for ATP formation via succinyl-CoA synthetase (SCS). Based on recent work on the ASCTs of F. hepatica, T. vaginalis and Trypanosoma brucei we suggest the existence of three subfamilies of enzymes within the CoA-transferase family I. Enzymes of these three subfamilies catalyse the ASCT reaction in eukaryotes via the same mechanism, but the subfamilies share little sequence homology. The CoA-transferases of the three subfamilies are all present inside ATP-producing organelles of parasites, those of subfamily IA in the mitochondria of trypanosomatids, subfamily IB in the mitochondria of parasitic worms and subfamily IC in hydrogenosome-bearing parasites. Together with the recent characterisation among non-parasitic protists of yet a third route of acetate formation involving acetate kinase (ACK) and phosphotransacetylase (PTA) that was previously unknown among eukaryotes, these recent developments provide a good opportunity to have a closer look at eukaryotic acetate formation.
International journal for parasitology 03/2010; 40(4):387-97. DOI:10.1016/j.ijpara.2009.12.006 · 3.87 Impact Factor
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ABSTRACT: This paper presents a modified genetic algorithm solution to the unit commitment problem (UCP), and constructs three kinds of genetic operators. To enhance convergence rate of the algorithm and prevent converging at a local optimal solution, a gene complementary technology is proposed and is applied to the modified genetic algorithm, which is called a gene complementary genetic algorithm (GCGA). Simulation results show that GCGA is a very efficient algorithm for solution to UCP
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