In vitro cytotoxic potential of Polyalthia longifolia on human cancer cell lines and induction of apoptosis through mitochondrial-dependent pathway in HL-60 cells
ABSTRACT Polyalthia longifolia is a lofty evergreen tree found in India and Sri Lanka. We are reporting first time the anticancer potential of P. longifolia leaves extract (A001) and its chloroform fraction (F002). Both inhibited cell proliferation of various human cancer cell lines in which colon cancer cells SW-620 showed maximum inhibition with IC(50) value 6.1 microg/ml. Furthermore, F002 induce apoptosis in human leukemia HL-60 cells as measured by several biological end points. F002 induce apoptotic bodies formation, DNA ladder, enhanced annexin-V-FITC binding of the cells, increased sub-G(0) DNA fraction, loss of mitochondrial membrane potential (DeltaPsi(mt)), release of cytochrome c, activation of caspase-9, caspase-3, and cleavage of poly ADP ribose polymerase (PARP) in HL-60 cells. All the above parameters revealed that F002-induced apoptosis through the mitochondrial-dependent pathway in HL-60 cells.
SourceAvailable from: Jorge Cadena-Iñiguez[Show abstract] [Hide abstract]
ABSTRACT: The antiproliferative potential of a crude extract from the chayote hybrid H-837-07-GISeM® and its potential for apoptosis induction were assessed in leukaemic cell lines and normal mouse bone marrow mononuclear cells (BM-MNCs). The extract strongly inhibited the proliferation of the P388, J774, and WEHI-3 cell lines (with an IC50 below 1.3 μg·mL(-1)), reduced cell viability, and induced apoptotic body production, phosphatidylserine translocation, and DNA fragmentation. However, the extract had no effect on BM-MNCs. We postulate that these properties make the extract a good candidate for an anti-tumour agent for clinical use.Nutrition and Cancer 01/2015; 67(2):1-8. DOI:10.1080/01635581.2015.989370 · 2.47 Impact Factor
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ABSTRACT: In this paper, we demonstrate the effect of dielectric barrier discharge (DBD) plasma on lymphoma and normal blood mononuclear cells. Results indicates enhanced level of reactive oxygen species in U937 cells, which presented a compromised redox status evident from increased NADP+/NADPH levels, reduced GSH/GSSG ratios and decreases in the total endogenous antioxidant activity of cells. A plasma treatment drastically reduces cell viability, causing more severe damage to DNA and mitochondria, as determined by means of apoptosis indicators and related genes expression. Taken together, these findings indicate a significant level of apoptosis in plasma-treated lymphoma cells. This report lends insight into progress toward an appropriate therapy for blood cancers using non-thermal DBD plasma.Plasma Processes and Polymers 11/2014; 11(12). DOI:10.1002/ppap.201400102 · 2.96 Impact Factor
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ABSTRACT: To screen the anticancer potential of the extracts of five Hainan plants of Polyalthia, which are also China Li minority ethnic medicines. The ethanol roots extracts of Polyalthia laui and Polyalthia rumphii inhibited proliferation of the selected four tumor cell lines at dose of 20 and 200 µg/ml demonstrating potential antitumor activities. Furthermore, the chloroform fraction and petroleum ether fractions of P. rumphii and P. laui exhibited cytotoxic effects against the four tumor cell lines and the IC 50 values ranged from 0.98 ± 0.3 µg/ml to 58.0 ± 3.5 µg/ml.