Collagen I gel can facilitate homogenous bone formation of adipose-derived stem cells in PLGA-beta-TCP scaffold
ABSTRACT Cell-based tissue engineering is thought to be a new therapy for treatment of bone defects and nonunions after trauma and tumor resection. In this study, we explore the in vitro and in vivo osteogenesis of a novel biomimetic construct fabricated by using collagen I gel to suspend rabbit adipose-derived stem cells (rASCs) into a porous poly(lactic-co-glycolic)acid-beta-tricalcium phosphate (PLGA-beta-TCP) scaffold (rASCs-COL/PLGA-beta-TCP). In vitro and in vivo studies of the rASCs-COL/PLGA-beta-TCP composite (group A) were carried out compared with the single combination of rASCs and PLGA-beta-TCP (rASCs/PLGA-beta-TCP; group B), the combination of acellular collagen I gel and PLGA-beta-TCP (COL/PLGA-beta-TCP; group C), and the PLGA-beta-TCP scaffold (group D). Composites of different groups were cultured in vitro for 2 weeks in osteogenic medium and then implanted into the autologous muscular intervals for 8 weeks. After 2 weeks of in vitro culture, alkaline phosphatase activity and extracellular matrix mineralization in group A were significantly higher than in group B (p < 0.01, n = 4). In vivo osteogenesis was evaluated by radiographic and histological analyses. The calcification level was radiographically evident in group A, whereas no apparent calcification was observed in groups B, C and D (n = 4). In group A, woven bone with a trabecular structure was formed, while in group B, only osteoid tissue was observed. Meanwhile, the bone-forming area in group A was significantly higher than in group B (p < 0.01, n = 4). No bone formation was observed in groups C or D (n = 4). In conclusion, by using collagen I gel to suspend rASCs into porous PLGA-beta-TCP scaffold, osteogenic differentiation of rASCs can be improved and homogeneous bone tissue can be successfully formed in vivo.
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- "Bone formation of adipose-derived stem cells M. Jiang et al. osteogenic differentiation of MSCs (Hao et al., 2008). In accordance with previous studies, higher proliferation rate and increased osteogenesis after combining opASCs with collagen I hydrogel in vitro occurred. "
ABSTRACT: To explore the osteogenic potency of adipose-derived stem cells from osteoporotic patients (opASCs). The opASCs were osteogenically induced in vitro with collagen I hydrogel or in culture plate. Detection of alkaline phosphatase (ALPase) and cell mineralization, and quantitative RT-PCR of collagen I, osteocalcin and bone sialoprotein were undertaken. Proliferation and morphology studies were also performed. After 14 days, opASCs-collagen I hydrogel composite was implanted into nude mice for 4 weeks prior to radiographic and histological analyses. Staining of ALPase and cell mineralization was strongly positive in opASCs. Fibroblast-like opASCs induced with collagen I hydrogel were evenly distributed and proliferated at a higher rate than in culture plates, showing similar growth curves for both genders. Expression of ALPase activity, cell mineralization and osteogenic-specific genes were higher in opASCs with collagen I hydrogel(male samples exhibited better osteogenicity than female samples) than in culture plates. After implantation for 4 weeks, radiopaque area signifying new bone tissue was observed in opASCs-collagen I hydrogel composite, with no donor gender differences. opASCs with collagen I hydrogel possesses adequate osteogenic potency and offers new opportunities for osteoporosis-related bone tissue engineering in male and female patients.Cell Biology International 01/2014; 38(1). DOI:10.1002/cbin.10182 · 1.64 Impact Factor
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- "In fact, in an ectopic environment no resident osteogenic cells are present and therefore the true potential of the implanted cells can be rigorously assessed. Only very few studies based on such ectopic models managed to demonstrate bone formation in vivo by human (hASC) or rabbit expanded ASC (Hattori et al., 2006; Scherberich et al., 2007; Hao et al., 2008; Jeon et al., 2008; Liu et al., 2008; Müller et al., 2009 "
ABSTRACT: The current need for bone grafts in orthopedic and reconstructive surgery cannot be satisfied by autologous tissue transplant due to its limited availability and significant associated morbidity. Tissue engineering approaches could supply sufficient amounts of bone substitutes by exploiting the ability to harvest autologous osteogenic progenitors associated with suitable porous materials. However, the generation of clinically relevant-sized constructs is critically hampered by limited vascularization, with consequent engraftment and survival only of a thin outer shell, upon in vivo implantation. To overcome this limitation, different non-mutually exclusive approaches have recently been developed to promote or accelerate graft vascularization, from angiogenic growth factor gene delivery to surgical pre-vascularization of the construct before implantation. A simple, promising strategy involves the co-culture of vasculogenic cells to form an intrinsic vascular network inside the graft in vitro, which can rapidly anastomose with the host blood vessels in vivo. Recent data have shown that adipose tissue-derived stromal vascular fraction (SVF) may provide an efficient, convenient, and autologous source for both osteogenic and endothelial cells. When SVF progenitors were cultured in appropriate bioreactor systems and ectopically implanted, a functional vascular network connected to the host was formed concomitantly to bone formation. Future studies should aim at demonstrating that this approach effectively supports survival of scaled up cell-based bone grafts at an orthotopic site. The procedure should also be adapted to become compatible with an intra-operative timeline and complemented with the definition of suitable potency markers, to facilitate its development into a simplified, reproducible, and cost-effective clinical treatment.Journal of Cellular Physiology 11/2010; 225(2):348-53. DOI:10.1002/jcp.22313 · 3.87 Impact Factor
Conference Paper: Implementing Ethernet in the industrial environment[Show abstract] [Hide abstract]
ABSTRACT: The media characteristics, topology, and noise of four Ethernet types, standard Ethernet, thin-coax Ethernet, broadband Ethernet, and twisted-pair Ethernet, are compared. Carrier sense multiple access with collision detection, the media access control method employed by Ethernet, is reviewed. Using the comparison results, different configurations of the network are examined to determine the advantages and disadvantages for each configuration, primarily noise susceptibility, reliability, and cost.< >Industry Applications Society Annual Meeting, 1990., Conference Record of the 1990 IEEE; 11/1990