Perinatal and postnatal exposure to bisphenol A increase adipose tissue mass and serum cholesterol level in mice

Department of Bone and Joint Surgery, Ehime University Graduate School of Medicine, Ehime, Japan.
Journal of atherosclerosis and thrombosis (Impact Factor: 2.77). 11/2007; 14(5):245-52. DOI: 10.5551/jat.E486
Source: PubMed

ABSTRACT To investigate whether the perinatal and postnatal exposure of mice to bisphenol A (BPA) caused the development of obesity and/or hyperlipidemia.
Pregnant mice were exposed to BPA in drinking water at concentrations of either 1 microg/mL (LD group) or 10 microg/mL (HD group) from gestation day 10 and throughout the lactating period. After weaning, the pups were allowed free access to drinking water containing the appropriate concentrations of BPA. The body weight, adipose tissue weight, and serum lipid levels were measured in the offspring at postnatal day 31.
In females, the mean body weight increased by 13% in the LD group (p<0.05) and 11% in the HD group (p<0.05) compared with the control group. The mean adipose tissue weight increased by 132% in the LD group (p<0.01). The mean total cholesterol level increased by 33% in the LD group (p<0.01) and 17% in the HD group (p<0.05). In males, the mean body weight and mean adipose tissue weight increased by 22% (p<0.01) and 59% (p<0.01), respectively, in the HD group compared with the control group. The mean triacylglycerol level increased by 34% in the LD group (p<0.05).
The continuous exposure of mice to BPA during the perinatal and postnatal periods caused the development of obesity and hyperlipidemia.

  • Source
    • "This profile included measurements of plasma glucose, cholesterol, and triglycerides . Obesity has been associated with hyperlipidemia in mice (Miyawaki et al., 2007). The fact that tail girth was increased in BPAtreated alligators relative to controls at weeks 3 and 5 suggests that BPA-treated neonates had more adipose tissue shortly after hatching "
    [Show abstract] [Hide abstract]
    ABSTRACT: Prenatal exposure to estrogenic endocrine disrupting chemicals (EDCs) can affect length of gestation and body mass and size of offspring. However, the dose, timing, and duration of exposure as well as sex and strain of the experimental animals determine the direction and magnitude of these effects. In this study, we examined the effects of a one-time embryonic exposure to either 17 β-estradiol (E2) or bisphenol A (BPA) on rate of development and growth in American alligators (Alligator mississippiensis). Our results indicate that BPA and E2-treated alligators hatched approximately 1.4days earlier than vehicle-treated (control) alligators, suggesting that estrogenic chemicals hasten hatching in these animals. We assessed growth rates, growth allometry, and body condition for 21weeks after hatching and found that BPA-treated alligators grew more quickly shortly after hatching but more slowly thereafter compared to control alligators. Conversely, E2-treated alligators grew more slowly shortly after hatching but more quickly thereafter compared to control alligators. As a result of differences in growth rate, BPA-treated alligators were heavier, longer, and fatter than control alligators at age 5weeks but were similar in size and leaner than control alligators at age 21weeks. Biochemical analytes were examined at the end of the 21-week study to assess overall metabolic condition. We found that E2-treated alligators had significantly higher circulating plasma concentrations of cholesterol and triglycerides than control alligators while BPA-treated alligators had blood profiles comparable to control alligators. Our results provide important insights into the effects of exogenous estrogens on morphology and metabolism in an oviparous, semi-aquatic reptile. Copyright © 2015 Elsevier Inc. All rights reserved.
    Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology 02/2015; 184. DOI:10.1016/j.cbpb.2015.02.001 · 1.90 Impact Factor
  • Source
    • "Additionally, the presence of such synthetic chemicals in humans has been associated with elevated triglycerides and cholesterol, impaired fasting glucose and diabetes (Meeker, 2012; Tang-Péronard et al., 2011), factors that are all related to the body's natural weight control mechanisms potentially leading to obesity. Exposure to EDCs, such as BPA and phthalates, has already been linked to obesity in animal studies (Miyawaki et al., 2007; R.R. Newbold et al., 2007). Such compounds are widely used as plasticizers and stabilizers in the manufacture of consumer products including children's toys and food-packaging materials (Koch and Calafat, 2009). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Bisphenol A (BPA) and triclosan (TCS) were determined in urine of Belgian overweight and obese (n = 151) and lean (n = 43) individuals. After the first urine collection (0 M), obese patients started a diet program or have undergone bariatric surgery. Hereafter, three additional urine samples from obese patients were collected after 3 (3M), 6 (6M) and 12 (12 M) months. Both compounds were detected in N99% of the samples. BPA had median concentrations of 1.7 and 1.2 ng/mL in obese and lean groups, respectively, while TCS had median concentrations of 1.5 and 0.9 ng/mL in the obese and lean groups, respectively. The obese group had higher urinary concentrations (ng/mL) of BPA (p b 0.5), while no significant differences were found for TCS between the obese and lean groups. No time trends between the different collection moments were observed. The BPA concentrations in the obese group were negatively associated with age, while no gender difference or relationship with body mass index was observed. For TCS, no relationships with gender, BMI, or age were found. The temporal variability of BPA and TCS was assessed with calculation of the intra class correlation coefficient, Spearman rank correlation coefficients, and surrogate category analysis. We observed evidence that single spot urine samples might be predictive of exposure over a longer period of time. Dietary intakes of BPA and TCS did not differ significantly among the time points considered after obese individuals started losing weight (6 and 12 months). Multiple linear regression analyses after adjusting for age and weight loss revealed negative associations between urinary TCS and serumFT4 in the 0M and 3M female obese individuals and positive associations between urinary BPA and serum TSH in the lean group.
    Environment international 01/2015; 76(March 2015):98-105. DOI:10.1016/j.envint.2014.12.003 · 5.66 Impact Factor
  • Source
    • "Most people worldwide have detectable levels of BPA in their blood or urine indicating a ubiquitous exposure (Vandenberg et al., 2007; Olsen et al., 2012). Furthermore, BPA has been shown to induce obesity in rodents in experimental settings by different groups (Miyawaki et al., 2007; Somm et al., 2009), and in humans. Lang et al. and Shankar et al. found urinary BPA to be related to obesity measured by BMI in the NHANES study performed in adults (Lang et al., 2008; Shankar et al., 2012). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Objective: Since bisphenol A (BPA) has been shown to induce obesity in experimental studies, we explored the associations between BPA and fat mass, fat distribution and circulating levels of adiponectin, leptin and ghrelin in humans. Methods: In the Prospective Investigation of the Vasculature in Uppsala Seniors (PIVUS), fat mass and fat distribution were determined in 70-year-old men and women (n = 890) by dual-energy X-ray absorptiometry (DXA) and magnetic resonance imaging (MRI) (n = 287). Serum levels of BPA were analyzed using isotope liquid chromatography/tandem mass spectrometer (API4000LC-MS/MS). Hormone levels were analyzed with radioimmunoassays (RIA) or enzyme-linked immunosorbent assay (ELISA). Imaging was performed approximately two years following collection of other data. Results: Serum concentrations of BPA were not related to adipose tissue measurements by DXA or MRI. BPA associated positively with adiponectin and leptin, but negatively with ghrelin, following adjustments for sex, height, fat mass, lean mass, smoking, alcohol consumption, physical activity, energy intake, and educational levels (p < 0.001, p = 0.009, p < 0.001, respectively). The relationship between BPA and ghrelin was stronger in women than in men. Conclusion: Although no relationships between BPA levels and measures of fat mass were seen, BPA associated strongly with the adipokines adiponectin and leptin and with the gut-hormone ghrelin suggesting that BPA may interfere with hormonal control of hunger and satiety. (C) 2014 Published by Elsevier Ltd.
    Chemosphere 10/2014; 112C:42-48. DOI:10.1016/j.chemosphere.2014.03.042 · 3.50 Impact Factor
Show more