Perinatal and Postnatal Exposure to Bisphenol A Increases Adipose Tissue Mass and Serum Cholesterol Level in Mice

Department of Bone and Joint Surgery, Ehime University Graduate School of Medicine, Ehime, Japan.
Journal of atherosclerosis and thrombosis (Impact Factor: 2.73). 11/2007; 14(5):245-52. DOI: 10.5551/jat.E486
Source: PubMed


To investigate whether the perinatal and postnatal exposure of mice to bisphenol A (BPA) caused the development of obesity and/or hyperlipidemia.
Pregnant mice were exposed to BPA in drinking water at concentrations of either 1 microg/mL (LD group) or 10 microg/mL (HD group) from gestation day 10 and throughout the lactating period. After weaning, the pups were allowed free access to drinking water containing the appropriate concentrations of BPA. The body weight, adipose tissue weight, and serum lipid levels were measured in the offspring at postnatal day 31.
In females, the mean body weight increased by 13% in the LD group (p<0.05) and 11% in the HD group (p<0.05) compared with the control group. The mean adipose tissue weight increased by 132% in the LD group (p<0.01). The mean total cholesterol level increased by 33% in the LD group (p<0.01) and 17% in the HD group (p<0.05). In males, the mean body weight and mean adipose tissue weight increased by 22% (p<0.01) and 59% (p<0.01), respectively, in the HD group compared with the control group. The mean triacylglycerol level increased by 34% in the LD group (p<0.05).
The continuous exposure of mice to BPA during the perinatal and postnatal periods caused the development of obesity and hyperlipidemia.

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    • "Offspring from rats exposed to BPA 0.1 mg/kg or 1.2 mg/kg from day 6 of pregnancy through the period of lactation showed an increase in body weight that resulted evident soon after birth and seemed to continue into adulthood (Rubin et al., 2001). Also, the BPA exposure from day 10 of gestation throughout the lactation period, but at lower doses (1 mg/mL or 10 mg/mL), resulted in an increment of adipose tissue and body weight (Miyawaki et al., 2007). Changes in the expression of some important adipogenic genes including PPAR-g (peroxisome proliferator activated receptor gamma), SREBP-1C (sterol regulatory element binding protein-1C), SCD-1 (stearoyl-CoA desaturase 1) and C/EBP-a (CCAAT/enhancer-binding protein alpha) have been also observed in female offspring at 21 days of age after perinatal exposure to 1 mg/L BPA (Somm et al., 2009). "
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    ABSTRACT: Obesity and type 2 diabetes mellitus (T2DM) are the most common metabolic disorders, with prevalence rates that are reaching epidemic proportions. Both are complex conditions affecting virtually all ages and with serious health consequences. The underlying cause of the problem is still puzzling, but both genetic and environmental factors including unhealthy diet, sedentary lifestyle, or the exposure to some environmental endocrine disrupting chemicals (EDCs) are thought to have a causal influence. In addition, the impact of early environment has recently emerged as an important factor responsible for the increased propensity to develop adult-onset metabolic disease. Suboptimal maternal nutrition during critical windows in fetal development is the most commonly studied factor affecting early programming of obesity and T2DM. In recent years, increasing experimental evidence shows that exposure to EDCs could also account for this phenomenon. In the present review, we will overview the most relevant findings that confirm the critical role of bisphenol-A, one of the most widespread EDCs, in the development of metabolic disorders.
    Dose-Response 06/2015; 13(2). DOI:10.1177/1559325815590395 · 1.22 Impact Factor
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    • "This profile included measurements of plasma glucose, cholesterol, and triglycerides . Obesity has been associated with hyperlipidemia in mice (Miyawaki et al., 2007). The fact that tail girth was increased in BPAtreated alligators relative to controls at weeks 3 and 5 suggests that BPA-treated neonates had more adipose tissue shortly after hatching "
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    ABSTRACT: Prenatal exposure to estrogenic endocrine disrupting chemicals (EDCs) can affect length of gestation and body mass and size of offspring. However, the dose, timing, and duration of exposure as well as sex and strain of the experimental animals determine the direction and magnitude of these effects. In this study, we examined the effects of a one-time embryonic exposure to either 17 β-estradiol (E2) or bisphenol A (BPA) on rate of development and growth in American alligators (Alligator mississippiensis). Our results indicate that BPA and E2-treated alligators hatched approximately 1.4days earlier than vehicle-treated (control) alligators, suggesting that estrogenic chemicals hasten hatching in these animals. We assessed growth rates, growth allometry, and body condition for 21weeks after hatching and found that BPA-treated alligators grew more quickly shortly after hatching but more slowly thereafter compared to control alligators. Conversely, E2-treated alligators grew more slowly shortly after hatching but more quickly thereafter compared to control alligators. As a result of differences in growth rate, BPA-treated alligators were heavier, longer, and fatter than control alligators at age 5weeks but were similar in size and leaner than control alligators at age 21weeks. Biochemical analytes were examined at the end of the 21-week study to assess overall metabolic condition. We found that E2-treated alligators had significantly higher circulating plasma concentrations of cholesterol and triglycerides than control alligators while BPA-treated alligators had blood profiles comparable to control alligators. Our results provide important insights into the effects of exogenous estrogens on morphology and metabolism in an oviparous, semi-aquatic reptile. Copyright © 2015 Elsevier Inc. All rights reserved.
    Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology 02/2015; 184. DOI:10.1016/j.cbpb.2015.02.001 · 1.55 Impact Factor
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    • "Additionally, the presence of such synthetic chemicals in humans has been associated with elevated triglycerides and cholesterol, impaired fasting glucose and diabetes (Meeker, 2012; Tang-Péronard et al., 2011), factors that are all related to the body's natural weight control mechanisms potentially leading to obesity. Exposure to EDCs, such as BPA and phthalates, has already been linked to obesity in animal studies (Miyawaki et al., 2007; R.R. Newbold et al., 2007). Such compounds are widely used as plasticizers and stabilizers in the manufacture of consumer products including children's toys and food-packaging materials (Koch and Calafat, 2009). "
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    ABSTRACT: Bisphenol A (BPA) and triclosan (TCS) were determined in urine of Belgian overweight and obese (n = 151) and lean (n = 43) individuals. After the first urine collection (0 M), obese patients started a diet program or have undergone bariatric surgery. Hereafter, three additional urine samples from obese patients were collected after 3 (3M), 6 (6M) and 12 (12 M) months. Both compounds were detected in N99% of the samples. BPA had median concentrations of 1.7 and 1.2 ng/mL in obese and lean groups, respectively, while TCS had median concentrations of 1.5 and 0.9 ng/mL in the obese and lean groups, respectively. The obese group had higher urinary concentrations (ng/mL) of BPA (p b 0.5), while no significant differences were found for TCS between the obese and lean groups. No time trends between the different collection moments were observed. The BPA concentrations in the obese group were negatively associated with age, while no gender difference or relationship with body mass index was observed. For TCS, no relationships with gender, BMI, or age were found. The temporal variability of BPA and TCS was assessed with calculation of the intra class correlation coefficient, Spearman rank correlation coefficients, and surrogate category analysis. We observed evidence that single spot urine samples might be predictive of exposure over a longer period of time. Dietary intakes of BPA and TCS did not differ significantly among the time points considered after obese individuals started losing weight (6 and 12 months). Multiple linear regression analyses after adjusting for age and weight loss revealed negative associations between urinary TCS and serumFT4 in the 0M and 3M female obese individuals and positive associations between urinary BPA and serum TSH in the lean group.
    Environment international 01/2015; 76(March 2015):98-105. DOI:10.1016/j.envint.2014.12.003 · 5.56 Impact Factor
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