Proteomic analysis of rice defense response induced by probenazole

Institute of Plant and Microbial Biology, Academia Sinica, 128, Sec. 2, Academia Road, Taipei 11529, Taiwan.
Phytochemistry (Impact Factor: 3.35). 03/2008; 69(3):715-28. DOI: 10.1016/j.phytochem.2007.09.005
Source: PubMed

ABSTRACT Here, we report the first proteomic analysis of rice defense response induced by probenazole (PBZ), an agricultural chemical that has been widely used to protect rice plants from rice blast and the bacterial blight pathogen. Two-dimensional gel electrophoresis (2-DE) was utilized to identify a total of 40 protein spots including 9 protein spots that are up-regulated by PBZ and 31 abundant protein spots. A total of 11 unique proteins from these 9 spots were identified by LC-MS/MS, and the majority of them were classified and/or possessed orthologs in defense-related functions. Five protein spots with only one protein species identified in each spot appear to be PBZ-regulated proteins. They are a putative glutathione S-transferase GSTU17, a putative phenylalanine ammonia-lyase (PAL, XP_466843), a putative caffeic acid 3-O-methyltransferase (COMT), a putative NADH-ubiquinone oxidoreductase, and a putative glucose-1-phosphate adenyltransferase. However, the other six protein species identified from the remaining four protein spots could not be conclusively described as PBZ-regulated proteins due to either the co-migration of two protein species in one spot or the presence of one protein species in two spots. Through real-time reverse transcription polymerase chain reaction (RT-PCR), it was determined that PAL (XP_466843) is likely regulated at the protein level, whereas GSTU17 and COMT were regulated at the mRNA level after PBZ application. Interestingly, the mRNA transcripts of two PAL paralogs were found to be up-regulated by PBZ. We propose that PAL, COMT, and GSTU17 are likely to confer PBZ-induced disease resistance via such functions as biosynthesis and transport of flavonoid-type phytoalexin and/or lignin biogenesis.

  • [Show abstract] [Hide abstract]
    ABSTRACT: Wheat powdery mildew, caused by Blumeria graminis f.sp. tritici (Bgt), is an important disease worldwide, causing significant yield losses annually. However, little is known about the proteomic response to powdery mildew infection in wheat. To analyse the leaf proteome changes of wheat in susceptible and resistant cultivars in response to Bgt, we compared the leaf proteins of susceptible cultivar Jingdong 8 (JD8) and its powdery mildew resistance near‐isogenic line (NIL) with a single Pm resistance gene Pm30 (JD8‐Pm30) at 0, 24 and 48 hours postinoculation (hpi) using a combination of two‐dimensional electrophoresis (2‐DE) and MALDI‐TOF MS. In total, 449 and 452 protein spots were reproducibly detected in leaves of JD8 and JD8‐Pm30, respectively, among which 53 (11.8%) and 44 (9.7%) were found to be polymorphic among 0, 24 and 48 hpi with the fold change of more than 1.5 and significant difference (P Bgt infection at translational level is different for the near‐isogenic lines. Moreover, 26 of the 97 differentially expressed proteins were identified, which included such functional categories as transcription and translation, energy and metabolism, signal transduction, disease and defence, as well as unclassified proteins. Results are discussed in terms of the functional implications of the proteins identified, with special emphasis on their putative roles in defence.
    Journal of Phytopathology 05/2012; 160(5). DOI:10.1111/j.1439-0434.2012.01889.x · 0.92 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Propamocarb is an agricultural chemical that has been widely used to protect cucumber plants from downy mildew. To understand the mechanisms of cucumber defense responses to propamocarb, we investigated the physiological and proteomic responses of the cucumber line D0351 with propamocarb application. We found that after treatment with propamocarb, the activities of detoxifying enzymes (glutathione reductase, GR; glutathione S-tramsferase, GST) and soluble sugar content of cucumber fruit were significantly increased, but malonaldehyde (MDA) content was significantly reduced. To identify components of propamocarb responsive signaling, we compared the high resolution two-dimensional gel electrophoresis (2-DE) protein profiles of control and propamocarb-treated fruits, and identified 18 differentially expressed (13 up-regulated and 5 down-regulated) proteins induced by propamocarb which were determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The majority of the proteins had functions related to detoxication, energy and transport, protein biosynthesis, regulating reactions and defending against stresses. A real-time quantitative reverse transcriptional-polymerase chain reaction (qRT-PCR) was used to compare transcript and protein accumulation patterns for 18 candidate proteins, and the expression of 14 was consistent at both transcript and protein levels. The responses of cucumber proteome to propamocarb seemed complex; the identified proteins may play an important role in regulating adaptation activities following exposure to propamocarb. Data presented herein may shed light on understanding cucumber fruit defense responses under propamocarb treatment.
    Journal of Integrative Agriculture 11/2013; 12(11):2022–2035. DOI:10.1016/S2095-3119(13)60370-6 · 0.63 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Atrazine is a photosystem-II-inhibiting herbicide that interferes with photosynthetic electron transport, resulting in oxidative stress. Soybean (Glycine max (L.) Merrill) is an atrazine-sensitive crop, and its productivity is severely impacted by soils containing atrazine residues. Our previous study indicated that the bacteria Klebsiella pneumoniae strain SnebYK-induced resistance to atrazine in soybean, both before and after pasteurization. In order to study the molecular mechanisms of this induced resistance, proteins change in soybean leaves induced by SnebYK was investigated using two-dimensional gel electrophoresis. Differentially expressed proteins (relative to a non-induced control) were identified using MALDI-TOF MS. Differential expression patterns were detected in soybean leaves that had been induced by the bacterium. Analysis of relative expression levels indicated up-regulation of most of the mRNAs in these samples relative to the control. The corresponding proteins were observed to be involved primarily in physiological processes, including active oxygen removal, resistance signal transduction, and photosynthesis. This is the first study to conduct proteomic analysis of a soybean resistance response induced by bacteria. It is plausible that these differentially expressed proteins may interact to play a major role in defense and/or resistance responses.
    Acta Physiologiae Plantarum 08/2014; 36(8):2229-2241. DOI:10.1007/s11738-014-1601-1 · 1.52 Impact Factor

Full-text (2 Sources)

Available from
Jun 2, 2014