Article

Optimization and validation of a high-performance liquid chromatographic method with UV detection for the determination of pyrrole-imidazole polyamides in rat plasma.

Department of Clinical Pharmacokinetics, College of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi, Chiba 274-8555, Japan.
Journal of Chromatography B (impact factor: 2.89). 12/2007; 859(2):272-5. DOI:10.1016/j.jchromb.2007.09.032 pp.272-5
Source: PubMed

ABSTRACT A simple and sensitive high-performance liquid chromatography (HPLC) method utilizing UV detection was developed for the determination of plasma pyrrole (Py)-imidazole (Im) polyamides in rats and applied to the pharmacokinetic study of compounds. After deproteinization of plasma with methanol, Py-Im polyamides were analyzed with a reversed-phase TSK-GEL ODS-80TM (4.6 mmx15.0 cm TOSOH Co., Japan) column maintained at 40 degrees C. The mobile phase solvent A was 0.1% acetic acid and the solvent B was HPLC-grade acetonitrile (0-10 min, A: 100-20%, B: 0-80% linear gradient; 10-15 min, A: 40%, B: 60%). The flow rate was 1.0 ml/min. The detection wavelength was set at 310 nm. The method was used to determine the plasma concentration time profiles of Py-Im polyamides after intravenous injection.

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Keywords

40 degrees C
 
intravenous injection
 
mobile phase solvent
 
plasma
 
plasma concentration time profiles
 
plasma pyrrole
 
Py)-imidazole
 
Py-Im polyamides
 
reversed-phase TSK-GEL ODS-80TM
 
sensitive high-performance liquid chromatography
 
solvent B